2012 Fiscal Year Final Research Report
Molecular switch mechanism for stop codon versatility by release factors (RF)
| Project/Area Number |
22310134
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
ITO Koichi 東京大学, 大学院・新領域創成科学研究科, 教授 (10262073)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 翻訳終結 / リボソーム / tRNA / 分子擬態 / ペプチド鎖解離因子 / 遺伝暗号解読 / タンパク質合成 |
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| Research Abstract |
Here we solved the first crystal structure of aRF1 and aPelota from an archaeon, Aeropyrum pernix (ape-aRF1) complexed with archaeal translation elongation factor aEF1α. Our biochemical and genetic analyses revealed that the authentic archaeal EF1α acts as a carrier GTPase for aRF1, and surprisingly, for aPelota, which functions in the mRNA surveillance pathways via tRNA mimicry. Our results revealed novel aspects of GTP switch mechanism for stop codon recognition.
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Research Products
(18 results)
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[Presentation] Omnipotent Role of Archaeal Elongation Factor 1 Alpha in Translation Elongation, and Termination, and Quality Control of Protein Synthesis2011
Author(s)
Kazuki Saito, Kan Kobayashi, Miki Wada,Izumi Kikuno, Akira Takusagawa, Masahiro Mochizuki, Toshio Uchiumi, Ryuichiro Ishitani,Osamu Nureki, Koichi Ito
Organizer
The 16th Annual Meeting of The RNA Society
Place of Presentation
Kyoto, Kyoto International Conference Center
Year and Date
20110612-18
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