2012 Fiscal Year Final Research Report

Evolution of the function maintenance system of plant organelle genome by C-to-U RNA editing

Research Project

Project/Area Number	22370002
Research Category	Grant-in-Aid for Scientific Research (B)
Allocation Type	Single-year Grants
Section	一般
Research Field	Genetics/Genome dynamics
Research Institution	Kyoto Prefectural University
Principal Investigator	OBOKATA Junichi 京都府立大学, 大学院・生命環境科学研究科, 教授 (50185667)
Co-Investigator(Kenkyū-buntansha)	SHIINA Takashi 京都府立大学, 生命環境科学研究科, 教授 (10206039) SUGIYAMA Yasuo 名古屋大学, 遺伝子実験施設, 准教授 (70154507)
Project Period (FY)	2010 – 2012
Keywords	植物オルガネラゲノム / 葉緑体RNA編集 / PPRタンパク質 / RNA分解酵素
Research Abstract	Catalytic molecules of the plant organellar RNA editing has long been unidentified. We attempted to isolate and characterize those molecules, using the psbE gene of Arabidopsis thaliana as a model. As a result, we succeeded in concentrating the catalytic activity from the chloroplast lysates by biochemical means, and found that RNase-sensitive component(s) should be involved in the catalytic reaction. Based on this we propose a tentative model of the C-to-U editing machinery.