2012 Fiscal Year Final Research Report
Evolution of the function maintenance system of plant organelle genome by C-to-U RNA editing
Project/Area Number |
22370002
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Genetics/Genome dynamics
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Research Institution | Kyoto Prefectural University |
Principal Investigator |
OBOKATA Junichi 京都府立大学, 大学院・生命環境科学研究科, 教授 (50185667)
|
Co-Investigator(Kenkyū-buntansha) |
SHIINA Takashi 京都府立大学, 生命環境科学研究科, 教授 (10206039)
SUGIYAMA Yasuo 名古屋大学, 遺伝子実験施設, 准教授 (70154507)
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Project Period (FY) |
2010 – 2012
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Keywords | 植物オルガネラゲノム / 葉緑体RNA編集 / PPRタンパク質 / RNA分解酵素 |
Research Abstract |
Catalytic molecules of the plant organellar RNA editing has long been unidentified. We attempted to isolate and characterize those molecules, using the psbE gene of Arabidopsis thaliana as a model. As a result, we succeeded in concentrating the catalytic activity from the chloroplast lysates by biochemical means, and found that RNase-sensitive component(s) should be involved in the catalytic reaction. Based on this we propose a tentative model of the C-to-U editing machinery.
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Research Products
(3 results)
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[Journal Article] Pentatricopeptide repeat proteins involved in plant organellar RNA editing2013
Author(s)
Yagi,Y., Tachikawa, M., Noguchi, H., Satoh, S., Obokata, Jand Nakamura, T
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Journal Title
RNA Biology
Volume: 10
Pages: 1-7
Peer Reviewed
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