Structural study of the Toll-like receptors signaling

2012 Fiscal Year Final Research Report

• Project/Area Number: 22370038
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Kyoto University
• Principal Investigator: TOCHIO Hidehito 京都大学, 大学院・工学研究科, 准教授 (70336593)
• Project Period (FY): 2010 – 2012
• Keywords: MyD88 / IRAK / 自然免疫 / Toll 様受容体 / NF-κB / IL-18

Research Abstract

The innate immune system is the self-defense system of higher eukaryotic organisms. The response of the system begins upon a recognition of pathogens such as bacteria and viruses by Toll-like Receptor(TLR) on the plasma membrane of immune cells. Once TLRs are activated by this recognition step, various cytosolic adaptor proteins such as MyD88 interact with the cytosolic region of TLRs to make a signal transduction complex. In this study, the interaction between adaptor proteins were studied. In addition, the receptor activation mechanism by Interlekin-18, the intracellular signal transduction pathway of which is shared with TLRs, were investigated.

Research Products

• [Journal Article] A Structuralized Monodisperse PEG Effectively Suppresses ProteinAggregation (2013)
  • Author(s): Muraoka, T., Adachi, K., Ui, M., Kawasaki, S., Sadhukhan, N., Obara, H., Tochio, H., Shirakawa, M., and Kinbara, K
  • Journal Title: Angewandte Chemie International Edition Volume: 52 Pages: 2430-4
  • DOI: doi:10.1002/anie.201206563
• [Journal Article] Real-time background-free selective imaging of fluorescent nanodiamonds in vivo (2012)
  • Author(s): Igarashi, R., Yoshinari, Y., Yokota, H., Sugi, T., Sugihara, F., Ikeda, K., Sumiya, H., Tsuji, S., Mori, I., Tochio, H., Harada, Y., Shirakawa, M
  • Journal Title: Nano Lett Volume: 12 Pages: 5726-32
  • DOI: doi:10.1021/nl302979d
• [Journal Article] Recognition of modification status on a histone H3 tail by linked histone reader modules of the epigenetic regulator UHRF1 (2012)
  • Author(s): Arita, K., Isogai, S., Oda, T., Unoki, M., Sugita, K., Sekiyama, N., Kuwata, K., Hamamoto, R., Tochio, H., Sato, M., Ariyoshi, M., Shirakawa, M
  • Journal Title: Proc Natl Acad Sci USA Volume: 109 Pages: 12950-12955
  • DOI: doi:10.1073/pnas.1203701109
• [Journal Article] TRAM is involved in IL-18 signaling and functions as a sorting adaptor for MyD88 (2012)
  • Author(s): Ohnishi, H., Tochio, H., Kato, Z., Kawamoto, N., Kimura, T., Kubota, K., Yamamoto, T., Funasaka, T., Nakano, H., Wong, R. W., Shirakawa, M., Kondo, N
  • Journal Title: PLoS One Volume: 7 Pages: e38423
  • DOI: doi:10.1371/journal.pone.0038423.
• [Journal Article] Molecular analysis of the binding mode of Toll/interleukin-1 receptor (TIR) domain proteins during TLR2 signaling (2012)
  • Author(s): Nada, M., Ohnishi, H., Tochio, H., Kato, Z., Kimura, T., Kubota, K., Yamamoto, T., Kamatari, Y. O.,Tsutsumi, N., Shirakawa, M., Kondo, N
  • Journal Title: Mol Immunol Volume: 52 Pages: 108-116
  • DOI: doi:10.1016/j.molimm.2012.05.003
• [Journal Article] NMR analysis of Lys63-linked polyubiquitin recognition by the tandem ubiquitin-interacting motifs of Rap80 (2012)
  • Author(s): Sekiyama, N., Jee, J., Isogai, S., Akagi, K., Huang, T. H., Ariyoshi, M., Tochio, H., Shirakawa, M
  • Journal Title: J Biomol N Volume: 52 Pages: 339-350
  • DOI: doi:10.1007/s10858-012-9614-9
• [Journal Article] Crystal structure of the ubiquitin-associated (UBA) domain of p62 and its interaction with ubiquitin (2011)
  • Author(s): Isogai S., Morimoto D., Arita K., Unzai S., Tenno T., Hasegawa J., Sou YS., Komatsu M., Tanaka K., Shirakawa M., Tochio H.
  • Journal Title: Journal of Biological Chemistry Volume: 286 Pages: 31864-31874
  • DOI: doi:10.1074/jbc.M111.259630
• [Journal Article] H-1, C-13, and N-15 resonance assignment of the TIR domain of human MyD88 (2010)
  • Author(s): Ohnishi H, Tochio H, Kato Z, Kimura T, Hiroaki H, Kondo N, Shirakawa M
  • Journal Title: Biomolecular NMR Assignments Volume: 4 Pages: 123-125
  • DOI: doi:10.1007/s12104-010-9222-0
• [Presentation] ヘムタンパク質 cytochrome cを用いた in-cell NMR 解析(口頭発表) (2012)
  • Author(s): 白井 隆弘, 猪股 晃介, 杤尾 豪人, 白川 昌宏
  • Organizer: 第35回分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121111-14
• [Presentation] UHRF2 の SRA ドメインによるメチル化 DNA 認識機構の解明 (ポスター発表) (2012)
  • Author(s): 村上 晃満, 有田 恭平, 有吉 眞理子, 杤尾 豪人, 白川 昌宏
  • Organizer: 第35回分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121111-14
• [Presentation] HP1αのフレキシブル N末端領域によるヒストン H3 結合の調節機構 (ポスター発表) (2012)
  • Author(s): 小宮 源之介, 大谷 淳二, 森本 大智, 星野 可奈子, 杤尾 豪人, 白川 昌宏, 有吉 眞理子
  • Organizer: 第35回分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 20121111-14
• [Presentation] ナノダイアモンドを用いた光検出磁気共鳴顕微鏡装置の開発と生物試料への応用 (口頭発表) (2012)
  • Author(s): 森 重之,吉成 洋祐,五十嵐 龍治,外間 進悟,横田 浩章,杤尾 豪人,白川 昌宏,原田 慶恵,池田 和寛,角谷 均
  • Organizer: 第51回NMR 討論
  • Place of Presentation: 名古屋
  • Year and Date: 20121108-10
• [Presentation] Recognition of interleukin-18 by the receptors studied by NMR spectroscopy (Posterpresentation) (2012)
  • Author(s): Naotaka Tsutsumi, Takeshi Kimura,Hidenori Ohnish, Zen-ichiro Kato, Hidehito Tochio, Naomi Kondo, Masahiro Shirakawa
  • Organizer: XXVth ICMRBS
  • Place of Presentation: Lyon, France
  • Year and Date: 20120819-24
• [Presentation] Magnetic resonance methods for analyses of structure and dynamics of intracellular proteins and cells (2011)
  • Author(s): Hidehito Tochio
  • Organizer: 第49回日本生物物理学会年会
  • Place of Presentation: 姫路
  • Year and Date: 20110916-18
• [Presentation] Analysis of proteins inside human cultured cells by NMR
  • Author(s): Hidehito Tochio
  • Organizer: The 4th Asia Pacific NMR symposium
  • Place of Presentation: Beijing, China.
  • Year and Date: 00001016-18
• [Book] 「ユビキチン鎖の多様性と認識機構」実験医学、29 巻(増刊号『タンパク質分解系による生体制御』) (2011)
  • Author(s): 磯貝信,杤尾豪人
  • Total Pages: 1915-1920
  • Publisher: 羊土社
• [Remarks]
  • URL: http://researchmap.jp/tochio/