Analysis of the outer membrane BAM complex

2012 Fiscal Year Final Research Report

• Project/Area Number: 22370044
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Structural biochemistry
• Research Institution: Yokohama City University
• Principal Investigator: J・R・H Tame 横浜市立大学, 生命医科学研究科, 教授 (00336588)
• Co-Investigator(Kenkyū-buntansha): PARK Sam-yong 横浜市立大学, 生命医科学研究科, 教授 (20291932) ; UNZAI Satoru 横浜市立大学, 生命医科学研究科, 助教 (60336592)
• Project Period (FY): 2010 – 2012
• Keywords: 膜タンパク質 / 抗生剤 / 結晶

Research Abstract

Gram negative bacteria require the BAM complex for the insertion of membrane proteins into the outer membrane. This essential complex consists of five proteins, the large transmembrane barrel being BamA. BamA carries five POTRA domains at its N terminus which interact with other proteins in the complex. Only BamA and BamD are essential, but the roles of these proteins is unknown. We have solved the crystal structure of an outer membrane protein, Hbp, and studied the interaction between BamC and BamD. The Hbp beta-barrel is a substrate of BAM, but the interactions between the two are unclear. We have shown that BamC and BamD interact to give a flexible complex.

Research Products

• [Journal Article] The structure of the deacetylase domain of Escherichia coli PgaB, an enzyme required for biofilm formation: a circularly permuted member of the carbohydrate esterase 4 family (2013)
  • Author(s): Nishiyama, T., Noguchi, H., Yoshida, H., Park, S. Y. & Tame, J. R. H
  • Journal Title: Acta Crystallogr D Biol Crystallogr Volume: 69 Pages: 44-51
• [Journal Article] Structural basis for broad detection of genogroup II noroviruses by a monoclonal antibody that binds to a site occluded in the viral particle (2012)
  • Author(s): Hansman, G. S., Taylor, D. W., McLellan,J. S., Smith, T. J., Georgiev, I., Tame, J. R. H., Park, S. Y., Yamazaki, M., Gondaira, F., Miki, M., Katayama, K., Murata, K. & Kwong, P. D
  • Journal Title: J Virol Volume: 86 Pages: 3635-46
• [Journal Article] Influence of structural symmetry on protein dynamics (2012)
  • Author(s): Matsunaga, Y., Koike, R., Ota, M., Tame, J. R. H. & Kidera, A
  • Journal Title: PLoS One Volume: 7 Pages: e50011
• [Journal Article] Structures of haemoglobin from woolly mammoth in liganded and unliganded states (2012)
  • Author(s): Noguchi, H., Campbell, K. L., Ho, C., Unzai, S., Park, S. Y. & Tame, J. R. H
  • Journal Title: Acta Crystallogr D Biol Crystallogr Volume: 68 Pages: 1441-9
• [Journal Article] Crystal structures of penicillin-binding protein 3 (PBP3) from methicillin-resistant Staphylococcus aureus in the apo and cefotaxime-bound forms (2012)
  • Author(s): Yoshida, H., Kawai, F., Obayashi, E., Akashi, S., Roper, D. I., Tame, J. R. H. & Park, S. Y.
  • Journal Title: J Mol Biol Volume: 423 Pages: 351-64
• [Journal Article] Crystal structure of unliganded TRAP:implications for dynamic allostery (2011)
  • Author(s): Malay, A. D., Watanabe, M., Heddle, J. G. & Tame, J. R. H
  • Journal Title: Biochem J Volume: 434 Pages: 427-34
• [Journal Article] Autotransporter protein secretion (2011)
  • Author(s): Tame, J. R. H
  • Journal Title: BioMol Concepts Volume: 2 Pages: 525-536
• [Journal Article] 4000 holes (2011)
  • Author(s): Tame, J. R. H
  • Journal Title: J Mol Biol Volume: 412 Pages: 551-2
• [Journal Article] Substitutions in woolly mammoth hemoglobin confer biochemical properties adaptive for cold tolerance (2010)
  • Author(s): Campbell, K. L., Roberts, J. E., Watson, L. N., Stetefeld, J., Sloan, A. M., Signore, A. V., Howatt, J. W., Tame, J. R. H.,Rohland, N., Shen, T. J., Austin, J. J., Hofreiter, M., Ho, C., Weber, R. E. & Cooper, A
  • Journal Title: Nat Genet Volume: 42 Pages: 536-40
• [Journal Article] Autotransporter passenger proteins: virulence factors with common structural themes (2010)
  • Author(s): Nishimura, K., Tajima, N., Yoon, Y. H., Park, S. Y. & Tame, J. R. H
  • Journal Title: J Mol Med Volume: 88 Pages: 451-8
• [Journal Article] Role of domains within the autotransporter Hbp/Tsh (2010)
  • Author(s): Nishimura, K., Yoon, Y. H., Kurihara, A., Unzai, S., Luirink, J., Park, S. Y. & Tame, J. R. H
  • Journal Title: Acta Crystallogr D Biol Crystallogr Volume: 66 Pages: 1295-300
• [Journal Article] Order and disorder in the domain organization of the plasmid partition protein KorB (2010)
  • Author(s): Rajasekar, K., Muntaha, S. T., Tame, J. R. H., Kommareddy, S., Morris, G., Wharton, C. W., Thomas, C. M., White, S. A., Hyde, E. I. & Scott, D. J
  • Journal Title: J Biol Chem Volume: 285 Pages: 15440-9
• [Journal Article] A conserved aromatic residue in the autochaperone domain of the autotransporter Hbp is critical for initiation of outer membrane translocation (2010)
  • Author(s): Soprova, Z., Sauri, A., van Ulsen, P., Tame, J. R. H., den Blaauwen, T., Jong, W. S. & Luirink, J.
  • Journal Title: J Biol Chem Volume: 285 Pages: 38224-33
• [Journal Article] A novel intein-like autoproteolytic mechanism in autotransporter proteins (2010)
  • Author(s): Tajima, N., Kawai, F., Park, S. Y. & Tame, J. R. H
  • Journal Title: J Mol Biol Volume: 402 Pages: 645-56
• [Presentation] Scoring functions (2012)
  • Author(s): Jeremy Tame
  • Organizer: CUP meeting
  • Place of Presentation: Santa Fe
  • Year and Date: 2012-03-07
• [Presentation] Drug design (2011)
  • Author(s): Jeremy Tame
  • Organizer: J-CUP II
  • Place of Presentation: Tokyo
  • Year and Date: 2011-05-20
• [Presentation] Animal hemoglobins (2010)
  • Author(s): Jeremy Tame
  • Organizer: Allosteric cooperativity. Bari University
  • Place of Presentation: Bari, Italy
  • Year and Date: 2010-06-01
• [Presentation] Influenza RNA polymerase (2010)
  • Author(s): Jeremy Tame
  • Organizer: RNA Society Meeting
  • Place of Presentation: Nottingham University, England
  • Year and Date: 2010-04-15
• [Presentation] Fifty years of globins (2009)
  • Author(s): Jeremy Tame
  • Organizer: European Crystallography Meeting
  • Place of Presentation: Istanbul, Turkey
  • Year and Date: 2009-08-21
• [Presentation] Thermodynamics and ligand binding (2009)
  • Author(s): Jeremy Tame
  • Organizer: Computer-aided drug design
  • Place of Presentation: Tilton, NH, USA.
  • Year and Date: 2009-06-24
• [Remarks]
  • URL: http://www-mls.tsurumi.yokohama-cu.ac.jp/pdl/index.html