2013 Fiscal Year Final Research Report
Regulatory mechanism of intracellular trafficking of ceramide
| Project/Area Number |
22370054
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | National Institute of Infectious Diseases |
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Principal Investigator |
HANADA KENTARO 国立感染症研究所, 細胞化学部, 部長 (30192701)
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| Co-Investigator(Renkei-kenkyūsha) |
KUMAGAI Keigo 国立感染症研究所, 細胞化学部, 主任研究官 (40443105)
YAMAJI Toshiyuki 国立感染症研究所, 細胞化学部, 主任研究官 (50332309)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 膜輸送 / 輸送タンパク質 / 脂質 / セラミド / スフィンゴミエリン / リン酸化 / 小胞体 / ゴルジ体 |
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| Research Abstract |
Lipid transfer proteins have been shown to mediate inter-organelle trafficking of various types of lipids in cells. However, mechanisms underlying the regulation of intracellular lipid trafficking remain poorly elucidated. In this research, we found i) phosphorylation at serine 315 of the CERT protein, which mediates transport of ceramide from the endoplasmic reticulum (ER) to the Golgi apparatus, strongly enhances the interaction of CERT with the ER-membrane protein VAP, and ii) this phosphorylation up-regulates ER-to-Golgi transport of ceramide. We previously showed that de-phosphorylation at another specific site of CERT up-regulates both activities for inter-membrane transfer of ceramide and Golgi-association of CERT. These results revealed that CERT-mediated ceramide trafficking is regulated by multiple protein kinase/phosphatase systems.
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[Journal Article] Limonoid compounds inhibit sphingomyelin biosynthesis by preventing CERT protein-dependent extraction of ceramides from the endoplasmic reticulum2012
Author(s)
Hullin-Matsuda F, Tomishige N, Sakai S, Ishitsuka R, Ishii K, Makino A, Greimel P, Abe M, Laviad EL, Lagarde M, Vidal H, Saito T, Osada H, Hanada K, Futerman AH, and Kobayashi T
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Journal Title
J Biol Chem
Volume: 287
Pages: 24397-24411
DOI
Peer Reviewed
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[Presentation] Structural basis for the preferential recognition of phosphatidylinositol 4-monophosphate by the pleckstrin homology domain of the ceramide trafficking protein CERT2012
Author(s)
Hanada K, Sugiki T, Takeuchi K, Tokunaga, Y, Terasawa, H, Kumagai, K, Yamaji, T, Takahashi, H, Shimada, I
Organizer
2012 ASBMB Special Symposium, Frontiers in Lipid Biology
Place of Presentation
Banff, Canada
Year and Date
20120904-09
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[Presentation] 抗がん性リモノイドはCERT による小胞体からのセラミドの引き抜きを阻害することによりスフィンゴミエリン合成を阻害する2011
Author(s)
Francoise Hullin-Matsuda,冨重斉生,牧野麻美,石塚玲子,石井久美子,Elad Lavee Laviad,Anthony Futerman,Michel Lagarde, Hubert Vidal,花田賢太郎,小林俊秀
Organizer
第6回スフィンゴテラピー研究会
Place of Presentation
米子
Year and Date
20110715-16
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