Study on channel protein gating dynamics by single molecule measurement.

2014 Fiscal Year Final Research Report

• Project/Area Number: 22370059
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Okayama University (2013-2014); The Graduate School for the Creation of New Photonics Industries (2011-2012); The Institute of Physical and Chemical Research (2010)
• Principal Investigator: IDE TORU 岡山大学, 自然科学研究科, 教授 (60231148)
• Project Period (FY): 2010-04-01 – 2015-03-31
• Keywords: １分子計測(SMD) / 生物物理学 / イオンチャネル

Outline of Final Research Achievements

In order for elucidating the structure-function relationship of ion-channel proteins, we have developed an apparatus for simultaneous optical and electrical measurement of single ion-channel proteins. Using this apparatus, we successfully measured single ligand bindings to single ion-channel proteins. We developed a novel method for measuring ion-channel current in the course of apparatus development process, which is applicable to ion-channel sensor devices. We revealed a relationship between the structure and the function of potassium channel, KcsA, using macroscopic methods.

Free Research Field

生物物理学