Studies on molecular mechanisms for radish male sterility/fertilityrestoration system and its diversification

2012 Fiscal Year Final Research Report

• Project/Area Number: 22380008
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Breeding science
• Research Institution: Kyoto Sangyo University
• Principal Investigator: TERACHI Toru 京都産業大学, 総合生命科学部, 教授 (90202192)
• Co-Investigator(Kenkyū-buntansha): YAMAGISHI Hiroshi 京都産業大学, 総合生命科学部, 教授 (10210345) ; KUROSAKA Akira 京都産業大学, 総合生命科学部, 教授 (90186536) ; TAKAHASHI Ryo 京都産業大学, 総合生命科学部, 助教 (50342811) ; NAKAMURA Takahiro 九州大学, 農学研究科, 准教授 (10464398) ; MIYAKE Keiko 岐阜大学, 男女共同参画推進室, 准教授 (40404058) ; KOIZUKA Nobuya 玉川大学, 農学部, 教授 (30433866)
• Project Period (FY): 2010 – 2012
• Keywords: ダイコン / 雄性不稔 / 稔性回復遺伝子 / PPR / ミトコンドリア

Research Abstract

In order to reveal molecular mechanisms underlying malesterility/fertility restoration in radish and the evolution of mitochondrial and nuclear genesrelevant to the phenomena, a series of experiments were conducted. Molecular studyrevealed that protein product from the gene pprB (one of the fertility restorer genes inradish) binds to mRNA of the mitochondrial male-sterility gene orf138 and inhibit itstranslation through the structural change of mRNA. Mitochondrial genomics revealedthat orf138 was created accidentally by the structural changes of mitochondrial genomesbetween normal and Ogura cytoplasm.

Research Products

• [Journal Article] Elucidation of the RNA recognition code for pentatricopeptide repeat proteins involved in organelle RNA editing in plants (2013)
  • Author(s): Yagi, Y., Hayashi, S., Kobayashi, K.,Hirayama, T.and Nakamura, T
  • Journal Title: PLoS ONE Volume: 8 Pages: e5728
  • Peer Reviewed
• [Journal Article] A complete mitochondrial genome sequence of Ogura-type male-sterile cytoplasm and its comparative analysis with that of normal cytoplasm in radish(Raphanus sativus L.) (2012)
  • Author(s): Tanaka, Y., Tsuda, M., Yasumoto, K.,Yamagishi, H. and Terachi, T
  • Journal Title: BMC Genomics Volume: 13 Pages: 352
  • DOI: doi:10.1186/1471-2164-13-352
  • Peer Reviewed
• [Journal Article] Mechanistic insight into pentatricopeptide repeat proteins as sequence-specific RNA-binding proteins for organellar RNAs in plants (2012)
  • Author(s): Nakamura, T., Yagi, Y. and Kobayashi, K
  • Journal Title: Plant Cell Physiol Volume: 53 Pages: 1171-1179
  • Peer Reviewed
• [Journal Article] Identification and characterization of the RNA binding surface of the pentatricopeptide repeat protein (2012)
  • Author(s): Kobayashi, K., Kawabata, M., Hisano, K.,Kazama, T., Matsuoka, K., Sugita, M. and Nakamura, T
  • Journal Title: Nucleic Acids Res Volume: 40 Pages: 2712-2723
  • Peer Reviewed
• [Journal Article] Polymorphic minisatellites in the mitochondrial DNAs of Oryza and Brassica (2011)
  • Author(s): Honma, Y., Yoshida, Y., Terachi, T.,Toriyama, K., Mikami, T. and Kubo, T
  • Journal Title: Current Genetics Volume: 57 Pages: 261-270
  • DOI: doi:10.1007/s00294-011-0345-3
  • Peer Reviewed
• [Presentation] オグラ型雄性不稔ダイコンの稔性回復に関わるRft遺伝子座の構造解析 (2013)
  • Author(s): 安本景太、高木宏樹、寺内良平、寺地徹、山岸博
  • Organizer: 日本育種学会第123回講演会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2013-03-27
• [Presentation] ダイコンミトコンドリアゲノムのプロモーター構造とゲノム組換えの関連性 (2013)
  • Author(s): 鳥塚悠太、今村順、肥塚信也
  • Organizer: 日本育種学会第123回講演会
  • Place of Presentation: 東京農業大学
  • Year and Date: 2013-03-27
• [Presentation] ダイコンミトコンドリアゲノムにコードされるatp8遺伝子座の転写開始点と転写後過程の解析 (2012)
  • Author(s): 鳥塚悠太、今村順、肥塚信也
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-09-15
• [Presentation] オルガネラゲノム工学による細胞質雄性不稔の開発 (2012)
  • Author(s): 山岸博
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-09-14
• [Presentation] ダイコンのミトコンドリアゲノムの多様性とミトコンドリアと相互作用する核遺伝子の進化 (2012)
  • Author(s): 寺地徹
  • Organizer: 日本育種学会第122回講演会
  • Place of Presentation: 京都産業大学
  • Year and Date: 2012-09-14
• [Presentation] ハマダイコンにおけるオグラ型雄性不稔に対する稔性回復遺伝子(ppr-B)の分子集団遺伝学的解析 (2012)
  • Author(s): 冨岡閲子、安本景太、平出美穂子、高橋亮、山岸博、寺地徹
  • Organizer: 日本育種学会第121回講演会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2012-03-30
• [Presentation] ダイコンのOS40型ミトコンドリアゲノムの構造解析-正常型およびオグラ型との比較 (2012)
  • Author(s): 田中義行、冨岡閲子、山岸博、寺地徹
  • Organizer: 日本育種学会第121回講演会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2012-03-30
• [Presentation] ダイコン細胞質雄性不稔遺伝子orf125を含む一次転写物の転写後過程の解析 (2012)
  • Author(s): 鳥塚悠太、今村順、肥塚信也
  • Organizer: 日本育種学会第121回講演会
  • Place of Presentation: 宇都宮大学
  • Year and Date: 2012-03-30
• [Presentation] Mitochondrial genome analysis of radish(Raphanus sativus) by next-generation sequencing (2011)
  • Author(s): Tanaka, Y., Tsuda, M., Yasumoto, K.,Yamagishi, H. and Terachi, T
  • Organizer: International Conference for Plant Mitochondrial Biology
  • Place of Presentation: Hohenroda, Germany
  • Year and Date: 2011-05-15
• [Presentation] A new fertility restorer gene for Ogura male-sterility found in wild radish (Raphanus raphanistrum L.) (2011)
  • Author(s): Yasumoto, K., Imamura, J., Koizuka, C.,Yamagishi, H. and Terachi, T
  • Organizer: International Conference for Plant Mitochondrial Biology
  • Place of Presentation: Hohenroda, Germany
  • Year and Date: 2011-05-15