Molecular mechamisms of specific interactions in bromovirus infections in rice

2012 Fiscal Year Final Research Report

• Project/Area Number: 22380030
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Plant pathology
• Research Institution: Kyoto University
• Principal Investigator: MISE Kazuyuki 京都大学, 大学院・農学研究科, 准教授 (90209776)
• Co-Investigator(Kenkyū-buntansha): OKUMOTO Yutaka 京都大学, 大学院・農学研究科, 教授 (90152438)
• Project Period (FY): 2010 – 2012
• Keywords: 遺伝学 / 遺伝子 / ウイルス / 植物 / 病理

Research Abstract

Specific interactions are observed in systemic infection of rice varieties with Brome mosaic virus (BMV) strains. We have identified rice and BMV factors that are involved in such interactions. Genetic analysis suggested that resistance responses of japonica rice varieties to BMV were controlled by a single dominant locus (600 kb region). Mutational analyses suggested that differences in systemic infection of rice with BMV strains were determined at the level of intercellular movement by several missense mutations within the C-terminal non-conserved region of BMV polymerase protein 2a.

Research Products

• [Journal Article] ADP ribosylation factor 1 plays an essential role in the replication of a plant RNA virus (2013)
  • Author(s): Hyodo, K., Mine, A., Taniguchi T., Kaido, M., Mise, K., Taniguchi, H., and Okuno, T
  • Journal Title: J. Virol Volume: 87(1) Pages: 163-176
• [Journal Article] Identification of domains in p27 auxiliary replicase protein essential for its association with the endoplasmic reticulum membranes in Red clover necrotic mosaic virus. (2012)
  • Author(s): Kusumanegara, K., Mine, A., Hyodo, K., Kaido, M., Mise, K., and Okuno, T
  • Journal Title: Virology Volume: 433(1) Pages: 131-141
  • DOI: DOI:10.1016/j.virol.2012.07.017
• [Journal Article] Poly(A)-binding protein facilitates translation of an uncapped/nonpolyadenylated viral RNA by binding to the 3´ untranslated region (2012)
  • Author(s): Iwakawa, H.-O., Tajima, Y., Taniguchi, T., Kaido, M., Mise, K., Tomari, Y., Taniguchi, H., and Okuno, T.
  • Journal Title: J. Virol Volume: 86(15) Pages: 7836-7849
  • DOI: DOI:10.1128/JVI.00538-12
• [Journal Article] Ef7 encodes an ELF3-like Protein and promotes rice flowering by negatively regulating the floral repressor gene Ghd7 under both short- and long-day conditions. (2012)
  • Author(s): Saito, H., Ogiso-Tanaka, E., Okumoto, Y., Yoshitake, Y., Izumi, H., Yokoo, T., Matsubara, K., Hori, K., Yano, M., Inoue, H., and Tanisaka, T
  • Journal Title: Plant Cell Physiol Volume: 53(4) Pages: 717-728
  • DOI: DOI:10.1093/pcp/pcs029
• [Journal Article] A long-distance RNA-RNA interaction plays an important role in programmed -1 ribosomal frameshifting in the translation of p88 replicase protein of Red clover necrotic mosaic virus (2011)
  • Author(s): Tajima, Y., Iwakawa, H.-O., Kaido, M., Mise, K., and Okuno, T
  • Journal Title: Virology Volume: 417(1) Pages: 169-178
  • DOI: DOI:10.1016/j.virol.2011.05.012
• [Journal Article] Identification of amino acids in auxiliary replicase protein p27 critical for its RNA-binding activity and the assembly of the replicase complex in Red clover necrotic mosaic virus (2011)
  • Author(s): Hyodo, K., Mine, A., Iwakawa, H.-O., Kaido, M., Mise, K., and Okuno, T
  • Journal Title: Virology Volume: 413(2) Pages: 300-309
  • DOI: DOI:10.1016/j.virol.2011.02.017
• [Journal Article] Viral cell-to-cell movement requires formation of cortical punctate structures containing Red clover necrotic mosaic virus movement protein (2011)
  • Author(s): Kaido, M., Funatsu, N., Tsuno, Y., Mise, K., and Okuno, T
  • Journal Title: Virology Volume: 413(2) Pages: 205-215
  • DOI: DOI:10.1016/j.virol.2011.02.008
• [Journal Article] Template recognition mechanisms by replicase proteins differ between bipartite positive-strand genomic RNAs of a plant virus (2011)
  • Author(s): Iwakawa, H.-O., Mine, A., Hyodo, K., An, M., Kaido, M., Mise, K., and Okuno, T.
  • Journal Title: J. Virol Volume: 85(1) Pages: 497-509
  • DOI: DOI:10.1128/JVI.01754-10
• [Journal Article] Complete loss of photoperiodic response in the rice mutant line X61 is caused by deficiency of phytochrome chromophore biosynthesis gene (2011)
  • Author(s): Saito, H., Okumoto, Y., Yoshitake, Y., Inoue, H., Yuan, Q., Teraishi, M., Tsukiyama, T., Nishida, H., and Tanisaka, T
  • Journal Title: Theor. Appl. Genet Volume: 122(1) Pages: 109-118
  • DOI: DOI:10.1007/s00122-010-1426-2
• [Journal Article] Suppression of a NAC-Like transcription factor gene improves boron-toxicity tolerance in rice. (2011)
  • Author(s): Mine, A., Ochiai, K., Shimizu, A., Okumoto, Y., Fujiwara, T., and Matoh, T
  • Journal Title: Plant Physiology Volume: 156(3) Pages: 1457-1463
  • DOI: DOI:10.1104/pp.110.171470
• [Journal Article] Interactions between p27 and p88 replicase proteins of Red clover necrotic mosaic virus play an essential role in viral RNA replication and suppression of RNA silencing via the 480-kDa viral replicase complex assembly. (2010)
  • Author(s): Hyodo, K., Takeda, A., Kaido, M., Mise, K., and Okuno, T
  • Journal Title: Virology Volume: 407(2) Pages: 213-224
  • DOI: DOI:10.1016/j.virol.2010.07.038
• [Journal Article] A Y-shaped RNA structure in the 3' untranslated region together with the trans-activator and core promoter of Red clover necrotic mosaic virusRNA2 is required for its negative-strand RNA synthesis (2010)
  • Author(s): An, M., Iwakawa, H.-O., Mine, A., Kaido, M., Mise, K., and Okuno, T
  • Journal Title: Virology Volume: 405(1) Pages: 100-109
  • DOI: DOI:10.1016/j.virol.2010.05.022
• [Journal Article] Identification and characterization of the 480 kDa template-specific RNA-dependent RNA polymerase complex of Red clover necrotic mosaic virus (2010)
  • Author(s): Mine, A., Takeda, A., Taniguchi, T., Taniguchi, H., Kaido, M., Mise, K., and Okuno, T.
  • Journal Title: J. Virol Volume: 84(12) Pages: 6070-6081
  • DOI: DOI:10.1128/JVI.00054-10
• [Journal Article] Differential roles of Hsp70 and Hsp90 in the assembly of the replicase complex of a positive-strand RNA plant virus
  • Author(s): Mine, A., Hyodo, K., Tajima, Y., Kusumanegara, K., Taniguchi, T., Kaido, M,Mise, K., Taniguchi, H., and Okuno, T.
  • Journal Title: J. Virol Volume: 86(22) Pages: 12091-12104
  • DOI: DOI:10.1128/JVI.01659-12
• [Presentation] Genetic analyses of incompatible interactions between bromoviruses and Arabidopsis thaliana. (2013)
  • Author(s): Mise
  • Organizer: Genetic analyses of incompatible interactions between bromoviruses and Arabidopsis thaliana.
  • Place of Presentation: Beijin, P. R. China
  • Year and Date: 20130825-30
• [Presentation] 2aタンパク質C末端非保存領域の2アミノ酸変異によってBrome mosaic virus(BMV)はDicer-like (DCL) 遺伝子欠損シロイヌナズナに全身感染する (2013)
  • Author(s): 矢崎ら
  • Organizer: 平成25年度日本植物病理学会大会
  • Place of Presentation: 岐阜市,岐阜大学
  • Year and Date: 2013-03-28
• [Presentation] A double-strand structure in the 5' untranslated region of Melandrium yellow fleck bromovirus RNA3 is involved in negative-strand RNA synthesis of RNA3. (2012)
  • Author(s): Narabayashi, T. et al
  • Organizer: 31th Annual Meeting of American Society for Virology
  • Place of Presentation: Madison, Wisconsin, USA
  • Year and Date: 20120721-25
• [Presentation] RNA3のコードおよび非コード領域の1塩基置換によってブロムモザイクウイルス (BMV)はDCL遺伝子欠損シロイヌナズナに全身感染する (2012)
  • Author(s): 矢崎ら
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡市,福岡国際会議場
  • Year and Date: 2012-03-29
• [Presentation] Melandrium yellow fleck bromovirus(MYFV) RNA3の5'非翻訳領域(UTR)に存在する2本鎖構造はRNA3のマイナス鎖合成に重要である (2012)
  • Author(s): 楢林ら
  • Organizer: 平成24年度日本植物病理学会大会
  • Place of Presentation: 福岡市,福岡国際会議場
  • Year and Date: 2012-03-29
• [Presentation] A transcripton-dependent translational regulation in the subgenomic RNA of Melandrium yellow fleck bromovirus (2011)
  • Author(s): Narabayashi., et al
  • Organizer: 15th International Congress of Virology
  • Place of Presentation: Sapporo, Japan.
  • Year and Date: 2011-09-12
• [Presentation] A novel regulation mechanism of viral gene expression via interaction between genomic and subgenomic RNAs of a plant virus. (2011)
  • Author(s): Narabayashi, T., et al
  • Organizer: 16th Annual Meeting of the RNA Society
  • Place of Presentation: Kyoto, Japan
  • Year and Date: 2011-06-17
• [Presentation] Melandrium yellow fleck virus (MYFV) RNA3の5'非翻訳領域(UTR)の変異はウイルス外被タンパク質 (CP) の翻訳に影響を与える (2011)
  • Organizer: 平成23年度日本植物病理学会大会
  • Place of Presentation: 府中市,東京農工大学
  • Year and Date: 2011-03-27
• [Presentation] 2a複製酵素タンパク質遺伝子内のミスセンス変異はBrome mosaic virus(BMV) のイネにおける全身感染能を付与する (2011)
  • Author(s): 新田ら
  • Organizer: 平成22年度日本植物病理学会大会,平成23年度日本植物病理学会大会
  • Place of Presentation: 府中市,東京農工大学
  • Year and Date: 2011-03-27
• [Presentation] Mutations in the 5' untranslated region of Melandrium yellow fleck bromovirus RNA3 affect translation of the coat protein gene. (2010)
  • Author(s): Narabayashi, T., et al
  • Organizer: 29th Annual Meeting of American Society for Virology
  • Place of Presentation: Bozeman, Montana, USA.
  • Year and Date: 20100717-21
• [Presentation] A novel regulation mechanism of viral gene expression via the interaction between genomic and subgenomic RNA (2010)
  • Author(s): 楢林ら
  • Organizer: 第33回日本分子生物学会年会
  • Place of Presentation: 神戸市,神戸国際会議場
  • Year and Date: 2010-12-09
• [Presentation] ゲノム-サブゲノム間相互作用による新規なウイルス遺伝子発現制御機構 (2010)
  • Author(s): 楢林ら
  • Organizer: 第58回日本ウイルス学会学術集会
  • Place of Presentation: 徳島市,あわぎんホール
  • Year and Date: 2010-11-09
• [Presentation] Melandrium yellow fleck virus(MYFV) RNA3の5'非翻訳領域(UTR)の変異はウイルス外被タンパク質 (CP) の翻訳に影響を与える (2010)
  • Author(s): 楢林ら
  • Organizer: 平成22年度日本植物病理学会大会
  • Place of Presentation: 京都市,国立京都国際会館
  • Year and Date: 2010-04-19
• [Remarks]
  • URL: http://www.plant-pathology.kais.kyoto-u.ac.jp/