2012 Fiscal Year Final Research Report
Development of a simple gene delivery system into insects by lipofection
| Project/Area Number |
22380039
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied entomology
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| Research Institution | National Institute of Agrobiological Sciences |
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Principal Investigator |
KAMIMURA Manabu 独立行政法人農業生物資源研究所, 昆虫成長制御研究ユニット, 主任研究員 (60370649)
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| Co-Investigator(Kenkyū-buntansha) |
MIYAMOTO Kazuhisa 独立行政法人農業生物資源研究所, 昆虫微生物機能研究ユニット, 上席研究員 (10370686)
KADONO Keiko 独立行政法人農業生物資源研究所, 昆虫ゲノム研究ユニット, 上席研究員 (40355722)
WADA Sanae 独立行政法人農業生物資源研究所, 昆虫微生物機能研究ユニット, 主任研究員 (50414959)
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| Co-Investigator(Renkei-kenkyūsha) |
SEZUTSU Hideki 独立行政法人農業生物資源研究所, 遺伝子組換えカイコ研究開発ユニット, ユニット長 (70342805)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 昆虫分子生物学 |
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| Research Abstract |
The firefly luciferase gene was successfully expressed by in vivo lipofection in more than 70 species of insects belonging to six orders. Expression of the E22O gene encoding an ecdysteroid inactivating enzyme by lipofection effectively decreased the 20-hydroxyecdysone titer and blocked molting in B. mori. Combining this gene delivery system with the luciferase assay revealed that the silkworm paralytic peptide is encoded by a polycistronic mRNA. These results indicate that in vivo lipofection can be used to analyze functions or expression control mechanisms of genes in varieties of insects.
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