Genomics of a diamondback moth and contribution to clarify the insecticide resistance mechanism

2012 Fiscal Year Final Research Report

• Project/Area Number: 22380041
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied entomology
• Research Institution: National Institute of Agrobiological Sciences
• Principal Investigator: YAMAMOTO Kimiko 独立行政法人農業生物資源研究所, 昆虫ゲノム研究ユニット, ユニット長 (40370689)
• Co-Investigator(Kenkyū-buntansha): MIYAMOTO Kazuhisa 農業生物資源研究所, 昆虫微生物機能研究ユニット, 上級研究員 (10370686) ; JOURAKU Akiya 農業生物資源研究所, 昆虫ゲノム研究ユニット, 任期付研究員 (60542115) ; SUETUGU Yoshitaka 農業生物資源研究所, 昆虫ゲノム研究ユニット, 主任研究員 (80533471) ; NODA Hiroaki 農業生物資源研究所, 昆虫科学研究領域, 特任上級研究員 (40343991) ; MITA Kazuei 農業生物資源研究所, 昆虫科学研究領域, 恃任上級研究員 (30159165)
• Project Period (FY): 2010 – 2012
• Keywords: 昆虫分子生物学

Research Abstract

EST analysis and RNAseq were performed and more than 30,000 gene candidates were acquired. KONAGAbase that provides diamondback moth comprehensive transcriptomic and draft genomic sequences with useful annotation information was developed and opened to the public. The candidates of Bt toxin resistance related genes were found with genetic analysis. The result of gene expression profiling showed that the genes associated with medicine metabolism had the low relevance with Bt toxin resistance.

Research Products

• [Journal Article] チョウ目害虫におけるBT剤およびBt毒素抵抗性の現状と原因遺伝子の同定 (2013)
  • Author(s): 宮本和久、渥美省吾、山本公子、瀬筒秀樹、野田博明
  • Journal Title: 植物防疫 Volume: 67(1) Pages: 27-34
  • Peer Reviewed
• [Presentation] The development of the genomic database of the diamondback moth (Plutella xylostella) and genomic analysis using it 6th Annual Arthropod Genomics Symposium: Arthropod Genomics (2013)
  • Author(s): Jouraku A, Yamamoto K, Kuwazaki S, Urio M, Suetsugu Y, Narukawa J, Miyamoto K, Kurita K, Kanamori H, Katayose Y, Matsumoto T, Noda H
  • Organizer: Taking Center Stage and i5k Community Workshop
  • Place of Presentation: Kansas City, USA
  • Year and Date: 2013-06-01
• [Presentation] KONAGAbase:agenomic database of the diamondback moth (2012)
  • Author(s): Jouraku A, Yamamoto K, Kuwazaki S, Urio M, Suetsugu Y, Narukawa J, Miyamoto K, Kurita K, Kanamori H, Katayose Y, Matsumoto T,Noda H
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡
  • Year and Date: 2012-12-12
• [Presentation] 殺虫剤抵抗性問題の最前線 (2012)
  • Author(s): 上樂明也
  • Organizer: 殺虫剤抵抗性問題におけるゲノム情報基盤の活用シンポジウム「ポストゲノム時代の害虫防除研究のあり方第5回」
  • Place of Presentation: 秋葉原
  • Year and Date: 2012-11-15
• [Presentation] Genome analysis of agricultural pests 2:Development of a genomic database of the diamondback moth and its application to genome analysis (2011)
  • Author(s): Jouraku A, Yamamoto K, Kuwazaki S, Urio M, Suetsugu Y, Narukawa J, Miyamoto K, Kurita K, Kanamori H, Katayose Y, Matsumoto T,Noda H
  • Organizer: 第34回日本分子生物学会年会
  • Place of Presentation: 横浜
  • Year and Date: 2011-12-14
• [Presentation] Current status of the development of the diamondback moth database (2011)
  • Author(s): Jouraku A, Yamamoto K, Kuwazaki S, Urio M, Suetsugu Y, Narukawa J, Miyamoto K, Kurita K, Kanamori H, Katayose Y, Matsumoto T, Noda H
  • Organizer: Sixth International Symposium on Molecular lnsect Science
  • Place of Presentation: アムステルダム、オランダ
  • Year and Date: 2011-10-04
• [Presentation] 農業害虫ゲノム解析-1.コナガゲノム塩基配列および発現遺伝子解析 (2010)
  • Author(s): 山本公子,上樂明也,桑崎誠剛,瓜尾政博,末次克行,生川潤子,宮本和久,野田博明
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-09
• [Presentation] Development of a genomic database of the diamondback moth (2010)
  • Author(s): Jouraku A, Yamamoto K, Kuwazaki S, Urio M, Suetsugu Y, Narukawa J, Miyamoto K, Noda H
  • Organizer: 第33回日本分子生物学会年会・第83回日本生化学会大会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 2010-12-08
• [Remarks]
  • URL: http://dbm. dna. affrc. go. jp/px/