2012 Fiscal Year Final Research Report
Molecular ecological study of dissemination and reproduction of tree pathogenic fungi
| Project/Area Number |
22380082
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Forest science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
GO Heiun 東京大学, 大学院・農学生命科学研究科, 助教 (10396814)
HOGETSU Taizo 東京大学, 大学院・農学生命科学研究科, 教授 (10107170)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 森林保護 / 植物病原体 / ナラ類集団枯損 / 腐朽病害 / 伝染環 / 胞子繁殖 / マイク ロサテライトマーカー / terminal RFLP法 |
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| Research Abstract |
To know the dispersal and reproductive mechanisms of tree pathogenic fungi, we developed polymorphic microsatellite markers of Raffaelea quercivora, Taphrina wiesneri, and Perenniporia fraxinea. Using these markers, we investigated the genetic structure of these fungi in seriously damaged sites. In addition, we investigated distribution of Armillaria tabescens, Ganoderma applanatum, and P. fraxinea within the trunk of infested trees using T-RFLP analysis. We also examined finer scale hypha distribution in the xylem of infested trees using hyphal staining with F-WGA.
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