2012 Fiscal Year Final Research Report
Development of micropropagation method by inducing embryogenic totipotent cells from non-pollen Cryptomeria
| Project/Area Number |
22380088
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Forest science
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| Research Institution | Forestry and Forest Products Research Institute |
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Principal Investigator |
ISHII Katsuaki 独立行政法人森林総合研究所, 森林バイオ研究センター, センター長 (80353572)
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| Co-Investigator(Kenkyū-buntansha) |
HOSOI Yoshihisa 森林総合研究所, 生物工学研究領域, チーム長 (50353842)
TANIGUCHI Toru 森林総合研究所, 森林バイオ研究センター, 室長 (00360470)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 森林生産 / 育種 / 組織培養 |
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| Research Abstract |
We developed the micropropagation technique using in vitro culture of non-pollen Cryptomeria (sugi) clones. Among the cytokinin tested, zeatin was relatively effective for bud induction. More buds were induced in the medium containing zeatin than that containing BAP with male sterile clone Fukushima 5. Shoots were developed from buds on the medium 1/2 LP containing 5 g/l activated charcoal. Shoots were rooted on the RIM medium containing 23.8 μM (5 mg/l) Cl-IAA and 0.044 μM (0.01 mg/l) BAP. These materials will be used for micropropagation of male sterile sugi cedar in order to reduce its pollen in the air.
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