Basic studies on the control of blood fluke infection of Pacific bluefin tuna

2012 Fiscal Year Final Research Report

• Project/Area Number: 22380105
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: 財団法人目黒寄生虫館 (2012); Meguro Parasitological Museum (2011); The University of Tokyo (2010)
• Principal Investigator: OGAWA Kazuo 財団法人目黒寄生虫館, 館長 (20092174)
• Co-Investigator(Kenkyū-buntansha): YOSHINAGA Tomoyoshi 東京大学, 農学生命科学研究科, 教授 (20345185) ; SAWADA Yoshifumi 近畿大学, 水産研究所, 教授 (80319764)
• Co-Investigator(Renkei-kenkyūsha): ISIMARU Katsuya 近畿大学, 水産研究所, 助教 (00330241) ; SHIRAKASHI Sho 近畿大学, 水産研究所, 講師 (70565936)
• Project Period (FY): 2010 – 2012
• Keywords: 住血吸虫 / クロマグロ / Cardicola / プラジクアンテル / 対策

Research Abstract

Firstly, a second blood fluke species from Pacific bluefin tuna was found from the heart ventricle and described as Cardicola opisthorchis n. sp. Secondly, new methods were developed to distinguish eggs of C. orientalis from those of C. opisthorchis morphologically, and to count the number of eggs in the gills. Thirdly, infection cycle was partly elucidated. Namely, both species of blood flukes were found in juvenile tuna 45 days after transfer to outside net cages; one week later, flukes were matured and laid eggs after one more week. Fourthly, C. opisthorchis was eradicated from tuna with oral administration of praziquantel (PZQ) at 7.5 mg/kg fish body weight or more for 3 consecutive days. Reinfection was confirmed 1 month after medication. PZQ concentration in tuna decreased to the undetectable level（0.02 μg/L）24 h after administration. Lastly, SEM observation of the two species of blood flukes shows swelling and contraction of the tegument and formation of many minute furrowed ridges in C. opisthorchis, while in C. orientalis, many minute holes were characteristically formed on the tegument.

Research Products

• [Journal Article] Praziquantel treatment against Cardicola blood flukes: determination of the minimal effective dose and pharmacokinetics in juvenile Pacific bluefin tuna (2013)
  • Author(s): Ishimaru, K., R. Mine, S. Shirakashi, E. Kaneko, K. Kubono, T. Okada, Y. Sawada and K. Ogawa
  • Journal Title: Aquaculture Volume: 402-403 Pages: 402-403,24-27
  • DOI: DOI:org/10.1016/j.aquaculture.2013.03.013
  • Peer Reviewed
• [Journal Article] Two species of Cardicola (Trematoda: Aporocotylidae) found in southern bluefin tuna, Thunnus maccoyii, ranched in South Australia. (2013)
  • Author(s): Shirakashi, S., K. Tsunemoto, C. Webber, K. Rough, D. Ellis and K. Ogawa
  • Journal Title: Fish Pathology Volume: 48 (1) Pages: 1-4
  • DOI: DOI: 10.3147/jsfp.48.1
  • Peer Reviewed
• [Journal Article] Oral treatment of praziquantel as an effective control measure against blood fluke infection in Pacific bluefin tuna (Thunnus orientalis (2012)
  • Author(s): Shirakashi, S., M. Andrews, Y. Kishimoto, K. Ishimaru, Y. Sawada, O. Murata and K. Ogawa
  • Journal Title: Aquaculture Pages: 326-329,15-19
  • DOI: DOI:10.1016/j.aquaculture.2011.10.035
  • Peer Reviewed
• [Journal Article] Morphology and distribution of blood fluke eggs and associated pathology in the gills of cultured Pacific bluefin tuna, Thunnus orientalis (2012)
  • Author(s): Shirakashi, S., Y. Kishimoto, R. Kinami, K. Katano, K. Ishimaru, O. Murata and K. Ogawa
  • Journal Title: Parasitology International Volume: 61 (2) Pages: 242-249
  • DOI: DOI: 10.1016/j.parint.2011.10.002
  • Peer Reviewed
• [Journal Article] Cardicola opisthorchis n. sp. (Trematoda: Aporocolidae) from Pacific bluefin tuna Thunnus orientalis (Temminck & Schlegel, 1844) cultured in Japan. (2011)
  • Author(s): Ogawa, K., K. Ishimaru, S. Shirakashi, I. Takami and D. Grabner
  • Journal Title: Parasitology International Volume: 60 (3) Pages: 307-312
  • DOI: DOI: 10.1016/j.parint.2011.05.002
  • Peer Reviewed
• [Presentation] Blood fluke infections of farmed Pacific bluefin tuna (2013)
  • Author(s): Ogawa, K
  • Organizer: ASP Parasitic Diseases in Fish Mariculture Workshop
  • Place of Presentation: Launceston, Tasmania, Australia
  • Year and Date: 2013-02-04
• [Presentation] オーストラリアの蓄養ミナミマグロにみられた住血吸虫の同定 (2012)
  • Author(s): 小川和夫・白樫 正・常本和伸・Claire Webber・Kirsten Rough
  • Organizer: 日本魚病学会春季大会
  • Place of Presentation: 山口県下関
  • Year and Date: 2012-09-16
• [Presentation] クロマグロ住血吸虫に対するプラジクアンテルの最低有効量と体内動態. (2012)
  • Author(s): 石丸克也,峰 龍馬,白樫 正,小川和夫,宮下 盛
  • Organizer: 日本魚病学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 2012-03-17
• [Presentation] Parasitic diseases of cultured Pacific bluefin tuna, Thunnus orientalis and development of control measure (2012)
  • Author(s): Shirakashi, S
  • Organizer: World Aquaculture Society
  • Place of Presentation: Las Vegas, U.S.A.
  • Year and Date: 2012-03-02
• [Presentation] クロマグロ住血吸虫に対するプラジクアンテルの有効性について (2011)
  • Author(s): 白樫 正・Melanie Andrews・石丸克也・ 岡田貴彦・村田 修・岸本喜樹・孫 暁楠・ 小川和夫
  • Organizer: 日本魚病学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20110300
• [Presentation] クロマグロ住血吸虫2 種の虫卵観察 (2011)
  • Author(s): 岸本喜樹・小川和夫・白樫正
  • Organizer: 日本魚病学会春季大会
  • Place of Presentation: 東京
  • Year and Date: 20110300
• [Presentation] Control of blood fluke infections in cultured Pacific bluefin tuna, Thunnus orientalis. (2011)
  • Author(s): Shirakashi, S., K. Ishimaru, T. Okada and K. Ogawa
  • Organizer: Eighth International Symposium on Fish Parasites
  • Place of Presentation: 東京
  • Year and Date: 2011-03-17
• [Presentation] 養殖クロマグロには2 種の住血吸虫が寄生する (2010)
  • Author(s): 小川和夫・白樫 正・石丸克也・村田 修
  • Organizer: 日本魚病学会秋季大会
  • Place of Presentation: 三重県津
  • Year and Date: 20100900
• [Patent(Industrial Property Rights)] 魚類の住血吸虫の駆除剤および魚類の住血吸虫の駆除方法 (2012)
  • Inventor(s): 小川和夫,村田修,澤田好史,石丸克也,白樫正,高見生雄,久保埜和成
  • Industrial Property Rights Holder: 国立大学法人東京大学,学校法人近畿大学,長崎県,あすか製薬株式会社
  • Industrial Property Number: 特願2012-009059,特開2012-162524
  • Filing Date: 2012-01-19