Role of chemokines in immune response of teleost fish

2012 Fiscal Year Final Research Report

• Project/Area Number: 22380112
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: General fisheries
• Research Institution: Nippon Veterinary and Life Science University
• Principal Investigator: KURATA Osamu 日本獣医生命科学大学, 獣医学部, 准教授 (90277666)
• Co-Investigator(Kenkyū-buntansha): MORITOMO Tadaaki 日本大学, 生物資源科学部, 准教授 (20239677) ; MATSUYAMA Tomomasa 独立行政法人水産総合研究センター, 研究員 (20372021) ; TAKANO Tomokazu 独立行政法人水産総合研究センター, 研究員 (40533998) ; SAKAI Takamitsu 独立行政法人水産総合研究センター, 研究員 (50416046)
• Project Period (FY): 2010 – 2012
• Keywords: ケモカイン / IL-8 / CXCL13 / 好中球 / Bリンパ球 / 炎症 / ヒラメ

Research Abstract

We revealed chemotactic activity of two types of chemokines, IL-8 and CXCL13 in Japanese flounder. IL-8 and CXCL13 induced selectively the migration of neutrophils and B lymphocytes, respectively. We also produced specific antibodies to these chemokines and developed immunoassays to estimate the quantity of these chemokines. Flounder leukocytes showed various responses in their IL-8 production that induced by stimulation with several species of bacterial pathogens. We suggested that IL-8 production is relevant to diversity of immune response observed in infectious diseases of Japanese flounder.

Research Products

• [Journal Article] Clonal growth of carp (Cyprinus carpio) T cells in vitro: Long-term proliferation of Th2-like cells. (2013)
  • Author(s): Yamaguchi, T., Katakura, F., Someya, K., Dijkstra, J. M., Moritomo, T. and Nakanishi, T.
  • Journal Title: Fish Shellfish Immunol. Volume: 34 Pages: 433-442
  • DOI: doi:10.1016/j.fsi.2012.11.005.
  • Peer Reviewed
• [Journal Article] Ontogeny of anti-viral hemorrhagic septicemia virus (VHSV) immunity in developing Japaneseflounder. (2012)
  • Author(s): Matsuyama, T., Nakayasu, C., Fujiwara,A., Kurita, Takano, T., Ito, T. and Sano, M.
  • Journal Title: Dev. Comp. Immunol. Volume: 37 Pages: 312-322
  • DOI: doi:10.1016/j.dci.2012.02.014.
  • Peer Reviewed
• [Journal Article] Suppression subtractive hybridization coupled with microarray analysis to examine differential expression of genes in Japanese flounder Paralichthys olivaceus leucocytes during Edwardsiella tarda and viral hemorrhagic septicemia virus infection. (2011)
  • Author(s): Matsuyama, T., Fujiwara, A., Takano, T.and Nakayasu, C.
  • Journal Title: Fish Shellfish Immunol. Volume: 31 Pages: 524-532
  • DOI: doi:10.1016/j.fsi.2011.06.015.
  • Peer Reviewed
• [Journal Article] Evaluating antigen-specific cytotoxicity of CD8+ T cells in fish by Granzyme B-likeactivity. (2011)
  • Author(s): Toda, H., Yabu, T., Shiba, H., Moritomo,T. and Nakanishi, T.
  • Journal Title: Vet. Immunol. Immunopathol. Volume: 141 Pages: 168-172
  • DOI: doi:10.1016/j.vetimm.2011.02.020.
  • Peer Reviewed
• [Journal Article] Perforin-dependent cytotoxic mechanism in killing by CD8 positive T cells in ginbuna crucian carp, Carassius auratus langsdorfii. (2011)
  • Author(s): Toda, H., Araki, K., Moritomo, T. andNakanishi, T.
  • Journal Title: Dev. Comp. Immunol. Volume: 35 Pages: 88-93
  • DOI: doi:10.1016/j.dci.2010.08.010.
  • Peer Reviewed
• [Journal Article] Protective efficacy of a formalin-killed vaccine against atypical Edwardsiella tarda infection in red sea bream Pagrus major. (2011)
  • Author(s): Takano, T. , Matsuyama, T., Sakai, T.,Nakayasu, C.
  • Journal Title: FishPathol. Volume: 46 Pages: 120-122
  • DOI: doi:10.3147/jsfp.46.120.
  • Peer Reviewed
• [Journal Article] Detection of Japanese flounder antibody against fimbrial major protein of Edwardsiella tarda. (2011)
  • Author(s): Sakai, T., Miyoshi, Y., Matsuyama, T.,Nakayasu, C., Kamaishi, T., Fukuda, Y. and Iida, T.
  • Journal Title: Fish Pathol. Volume: 46 Pages: 23-26
  • DOI: doi:10.3147/jsfp.46.23.
  • Peer Reviewed
• [Journal Article] Lymphocytes with T-cell-like properties express the Fas ligand in the Japanese flounder Paralichthys olivaceus. (2011)
  • Author(s): Kurata, O., Iwasaki, T., Matsuyama, T.,Nakayasu, C., Wada, S. and Hatai, K.
  • Journal Title: Fish Shellfish Immunol. Volume: 30 Pages: 509-514
  • DOI: doi:10.1016/j.fsi.2010.11.030.
  • Peer Reviewed
• [Journal Article] Long-term culture technology for Japanese flounder Paralichthys olivaceus leukocytes. (2011)
  • Author(s): Ozaki, T., Hatakeyama, H., Wada, S. andKurata, O.
  • Journal Title: Fish Pathol. Volume: 46 Pages: 11-18
  • DOI: doi:10.3147/jsfp.46.11.
  • Peer Reviewed
• [Journal Article] Activity of granulocytes and chemokines in the leukocyte- encapsulation response of Japanese flounder Paralichthys olivaceus. (2010)
  • Author(s): Kurata, O., Kitancharoen, N., Fujiwara, A., Nakayasu, C., Wada, S. and Hatai, K.
  • Journal Title: Fish Pathol. Volume: 45 Pages: 121-129
  • DOI: doi:10.3147/jsfp.45.121.
  • Peer Reviewed
• [Journal Article] Immunocytochemical studies of the ontogeny of peripheral blood leucocyte subpopulations in Japanese flounder (Paralichthys olivaceus). (2010)
  • Author(s): Matsuyama, T., Nakayasua, C. and Sano,M.
  • Journal Title: FishShellfish Immunol. Volume: 29 Pages: 362-365
  • DOI: doi:10.1016/j.fsi.2010.03.005.
  • Peer Reviewed
• [Journal Article] The efficacy of five avirulent Edwardsiella tarda strains in a live vaccine against Edwardsiellosis in Japanese flounder, Paralichthys olivaceus. (2010)
  • Author(s): Takano, T., Matsuyama, T., Oseko, N.,Sakai, T., Kamaishi, T., Nakayasu, C., Sano, M., Iida, T.
  • Journal Title: FishShellfish Immunol. Volume: 29 Pages: 687-693
  • DOI: doi:10.1016/j.fsi.2010.07.012.
  • Peer Reviewed
• [Journal Article] Demonstration of T cell and macrophage progenitors in carp (Cyprinus carpio) kidney hematopoietic tissues. (2010)
  • Author(s): Katakura, F., Yamaguchi, T., Yoshida,M., Moritomo, T. and Nakanishi, T.
  • Journal Title: Dev. Comp. Immunol. Volume: 34 Pages: 685-689
  • DOI: doi:10.1016/j.dci.2010.01.015.
  • Peer Reviewed
• [Journal Article] Clonal growth of carp (Cyprinus carpio) T cells in vitro. (2010)
  • Author(s): Yamaguchi, T., Katakura, F., Shitanda,S., Niida, Y., Toda, H., Ohtani, M., Yabu, T., Suetake, H., Moritomo, T. andNakanishi, T.
  • Journal Title: Dev. Comp. Immunol. Volume: 35 Pages: 193-202
  • DOI: doi:10.1016/j.dci.2010.09.007.
  • Peer Reviewed
• [Journal Article] Comparative gene expression analysis of zebrafish and mammals identifies common regulators in hematopoieticstem cells. (2010)
  • Author(s): Kobayashi, I., Ono, H., Moritomo, T.,Kano, K., Nakanishi, T. and Suda, T.
  • Journal Title: Blood Volume: 115 Pages: e1-e9
  • DOI: dOI:10.1182/blood-2009-07-232332.
  • Peer Reviewed
• [Presentation] ヒラメIL-8タンパク検出のための特異抗体の作製 (2013)
  • Author(s): 倉田修
  • Organizer: 平成25年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2013-03-29
• [Presentation] 好中球の走化性に関与するヒラメIL-8のN末領域 (2012)
  • Author(s): 倉田修
  • Organizer: 平成24年度日本水産学会秋季大会
  • Place of Presentation: 独立行政法人水産大学校
  • Year and Date: 2012-09-15
• [Presentation] N-terminal region responsible for chemotactic activity of flounder IL-8. (2012)
  • Author(s): Kurata, O.
  • Organizer: The 12th congress of ISDCI
  • Place of Presentation: Hilton Fukuoka Sea Hawk Hotel.
  • Year and Date: 2012-07-10
• [Presentation] ヒラメCXCケモカインに対する白血球走化性について (2011)
  • Author(s): 倉田修
  • Organizer: 平成23年度日本魚病学会秋季大会
  • Place of Presentation: 長崎大学文教キャンパス
  • Year and Date: 2011-10-02
• [Presentation] 支持細胞上で増殖するヒラメ白血球の起源. (2011)
  • Author(s): 倉田修
  • Organizer: 平成23年度日本水産学会秋季大会
  • Place of Presentation: 長崎大学文教キャンパス
  • Year and Date: 2011-10-01
• [Presentation] Immune-related gene expression in leukocyte subpopulations of Japanese flounder. (2011)
  • Author(s): Takano, T.
  • Organizer: The 15th International Conference on Diseases of Fish and Shellfish
  • Place of Presentation: Split, Croatia.
  • Year and Date: 2011-09-14
• [Presentation] ヒラメCXCケモカインの機能解析. (2011)
  • Author(s): 倉田修
  • Organizer: 平成23年度日本水産学会春季大会
  • Place of Presentation: 東京海洋大学
  • Year and Date: 2011-03-28