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2012 Fiscal Year Final Research Report

Construction of anti-ulcerative colitis (UC) yogurt by probiotic blood type lactic acid bacteria

Research Project

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Project/Area Number 22380144
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section一般
Research Field Zootechnical science/Grassland science
Research InstitutionTohoku University

Principal Investigator

SAITO Tadao  東北大学, 大学院・農学研究科, 教授 (00118358)

Co-Investigator(Kenkyū-buntansha) KITAZAWA Haruki  東北大学, 大学院・農学研究科, 准教授 (10204885)
KAWAI Yasushi  東北大学, 大学院・農学研究科, 助教 (00261496)
NISHIMURA Junko  東北大学, 大学院・農学研究科, 技術専門職員 (10241556)
Project Period (FY) 2010 – 2012
Keywords乳酸菌 / プロバイオティクス / 血液型乳酸菌 / 潰瘍性大腸炎 / 競合阻害 / ビフィ ズス菌 / アドヘシン / 機能性ヨーグルト
Research Abstract

A new evaluation system to select probiotic lactic acid bacteria (LAB) with adherence to human colonic mucin (HCM) by recognizing sialic acid and/or surfate on ABO-blood type antigens. LAB strains showing strong adhesion to human blood type-A antigen expressed on the intestinal mucosa were selected from 237 probiotic strains using the biosensor BIACORE. A similar method was used to select harmful bacteria that also recognizing the same antigen in the human intestine. Fusobacterium varium was detected as one of harmful bacteria from the patient of ulcerative colitics. Using a competitive adhesion experiment with a micro plate coated with the type-A antigen and the harmful bacteria, the bacteria was significantly decreased when they were added in combination with the type-A LAB. Our data suggested the blood type LAB strains may competitively inhibit pathogenic infections where this strategy may be useful in the development of improved future probiotic foods to protect human health.

  • Research Products

    (21 results)

All 2012 2011 2010

All Journal Article (8 results) (of which Peer Reviewed: 6 results) Presentation (13 results)

  • [Journal Article] Characterization of lactose utilization and beta-galactosidase in Lactobacillus brevis KB290, thehetero-fermentative lactic acid bacterium2012

    • Author(s)
      Honda, H., N.Yajima and T. Saito
    • Journal Title

      Curr. Microbiol

      Volume: 65 Pages: 679-685

    • DOI

      DOI:10.1007/s00284-012-0216-2

    • Peer Reviewed
  • [Journal Article] New screening methods for probiotics with adhesion properties to sialic acid and sulphate residues in human colonic mucin using the Biacore assay2012

    • Author(s)
      Huang, I-N., T. Okawara, M. Watanabe, Y. Kawai, H. Kitazawa, S. Ohnuma, C. Shibata, A. Horii, K. Kimura, N. Taketomo, J-Z. Xiao, K. Iwatsuki and T. Saito
    • Journal Title

      J. Appl. Microbiol

      Volume: 114 Pages: 854-860

    • DOI

      DOI:10.1111/jam.12063

    • Peer Reviewed
  • [Journal Article] Purification and characterization of two phosphor-beta -galactosidases, LacG1 and LacG2, from Lactobacillus gasseri ATCC33323T2012

    • Author(s)
      Honda, H., S. Nagaoka, Y. Kawai, R.Kemperman, J. Kok, Y. Yamazaki, Y. Tateno, H. Kitazawa and T. Saito
    • Journal Title

      J. Gen. Appl. Microbiol

      Volume: 58 Pages: 11-17

    • Peer Reviewed
  • [Journal Article] An adhesin-like protein, Lam29, from Lactobacillus mucosae ME-340 binds to histone H3 and blood group antigens in human colonic mucus2012

    • Author(s)
      Watanabe, M., H. Kinoshita, I-N. Huang, K. Eguchi, T. Tsurumi, Y. Kawai, H. Kitazawa, K. Kimura, N. Taketomo, D. Kikuchi, T. Sase, K. Miura,H. Ogawa, C. Shibata, A. Horii and T. Saito
    • Journal Title

      Biosci. Biotechnol. Biochem

      Volume: 76 Pages: 1655-1660

    • DOI

      DOI:10.1271/bbb.120150

    • Peer Reviewed
  • [Journal Article] 「乳酸菌」プロバイオティッ ク乳酸菌のヒト腸管付着性機構の解明と応用2012

    • Author(s)
      齋藤忠夫
    • Journal Title

      New Diet Therapy

      Volume: 27 Pages: 13-22

  • [Journal Article] プロバイオティクスの評価システム構築と製品化への新展開2012

    • Author(s)
      齋藤忠夫
    • Journal Title

      食品と開発

      Volume: 48 Pages: 8-11

  • [Journal Article] Oral administration of phosphorylated dextran regulates immune response in ovalbumin- immunized mice2010

    • Author(s)
      Nagasawa, C., Nishimura-Uemura, J., Tohono, M., T. Shimosato, Y. Kawai, S. Ikegami, M. Oda, T. Saito and H.Kitazawa
    • Journal Title

      Asian-Aust. J. Anim. Sci

      Volume: 23 Pages: 106-115

    • Peer Reviewed
  • [Journal Article] Identification of a new adhesin-like protein form Lactobacillus mucosae ME-340 with specific affinity to the human blood group A and B antigens2010

    • Author(s)
      Watanabe, M., H. Kinoshita, N. Nitta, R. Yukishita, Y. Kawai, K Kimura, N. Taketomo, Y. Yamazaki, Y. Tateno, K. Miura, A. Horii, H. Kitazawa, T. Saito
    • Journal Title

      J. Appl. Microbiol

      Volume: 109 Pages: 927-935

    • DOI

      DOI:10.1111/j.1365-2672.2010.04719.x

    • Peer Reviewed
  • [Presentation] ヒト腸内細菌の大腸ムチン結合部位決定のための酸性基に注目した新しいBiacore評価系の構築2012

    • Author(s)
      黄懿儂、渡辺真通、大川原崇、川井泰、北澤春樹、大沼忍、柴田近、堀井明、木村勝紀、竹友直生、齋藤忠夫
    • Organizer
      日本乳酸菌学会2012年度大会、つくば国際会議場
    • Place of Presentation
      つくば
    • Year and Date
      20120712-13
  • [Presentation] New Functional Yogurts as FOSHU and Their Evaluation System in Japan2012

    • Author(s)
      Saito, T
    • Organizer
      The 7th International Symposium on Lactic Acid Bacteria and Health , The 3rd Asian Symposium on Lactic Acid Bacteria
    • Place of Presentation
      Wuxi, China
    • Year and Date
      20120529-31
  • [Presentation] New evaluation system using Biacore to select probiotics with high binding activity to acidic residues of human colonic mucin2012

    • Author(s)
      Huang, I. N., T. Okawara, M. Watanabe, Y. Kawai, H. Kitazawa, S. Ohnuma, C. Shibata, A. Horii, T. Saito
    • Organizer
      The 7th International Symposium on Lactic Acid Bacteria and Health, The 3rd Asian Symposium on Lactic Acid Bacteria
    • Place of Presentation
      Wuxi, China
    • Year and Date
      20120529-31
  • [Presentation] 潰瘍性大腸炎(UC)の炎症緩和効果を示す血液型乳酸菌に関する基盤的研究(その1)2012

    • Author(s)
      齋藤忠夫、佐藤優理、坂本光央、大熊盛也、小川仁、三浦康、柴田近、川井泰、北澤春樹、堀井明
    • Organizer
      日本畜産学会第115回大会、名古屋大学
    • Place of Presentation
      名古屋
    • Year and Date
      20120328-30
  • [Presentation] Lactobacillus mucosae由来アドへシン様タンパク質(Lam29)の新規ヒト大腸粘液由来結合因子の探索および同定2012

    • Author(s)
      渡辺真通、木下英樹、川井泰、北澤春樹、三浦康、柴田近、堀井明、木村勝紀、竹友直生、齋藤忠夫
    • Organizer
      日本畜産学会第115回大会、名古屋大学
    • Place of Presentation
      名古屋
    • Year and Date
      20120328-30
  • [Presentation] プロバイオティック乳酸菌のヒト大腸ムチンにおけるシアル酸・硫酸基結合評価系のBiacoreによる新構築2012

    • Author(s)
      黄懿儂、春田美由樹、渡辺真通、江口慧、鶴見卓也、大川原崇、川井泰、北澤春樹、大沼忍、柴田近、堀井明、木村勝紀、竹友直生、齋藤忠夫
    • Organizer
      日本畜産学会第115回大会、名古屋大学
    • Place of Presentation
      名古屋
    • Year and Date
      20120328-30
  • [Presentation] 潰瘍性大腸炎の炎症緩和効果を有するプロバイオティクスに関する基盤的研究(その1)2012

    • Author(s)
      佐藤優理、坂本光央、小川仁、三浦康、柴田近、川井泰、北澤春樹、大熊盛也、堀井明、齋藤忠夫
    • Organizer
      日本農芸化学会2012年度大会、京都女子大学
    • Place of Presentation
      京都
    • Year and Date
      20120324-27
  • [Presentation] Lactic Acid Bacteria and Dairy Foods, Inaugural Worldwide Symposium on Milk and Human Health2012

    • Author(s)
      Saito, T
    • Organizer
      Organized by Korean Society for Lactic Acid Bacteria, Shilla Hotel, Seoul
    • Place of Presentation
      KOREA
    • Year and Date
      2012-11-08
  • [Presentation] Immunogenics and survival strategy of Lactobacillus GG in human gut2012

    • Author(s)
      Saito, T
    • Organizer
      The 3rd International Symposium on Lactobacillus GG of the Korean Society for Lactic Acid Bacteria (KSLAB), Grand Ballroom, The Hotel Plaza Seoul
    • Place of Presentation
      Seoul City, Korea
    • Year and Date
      2012-05-09
  • [Presentation] Future Strategy of New Yogurts Using Adhesive Probiotic Lactic Acid Bacteria (LAB) to Protect Our Gut from Inflammatory Bowel Disease (IBD). LAB6-6, Session6, Technologies and Applications2011

    • Author(s)
      Saito, T
    • Organizer
      The 6th Asian Conference on Lactic Acid Bacteria. XII International Congress of Bacteriology and Applied Microbiology (ACLAB6), Organized by Japan Society for Lactic Acid Bacteria (JSLAB) and Asian Federation of Societies for Lactic Acid Bacteria (AFSLAB), Sapporo Convention Center & Sapporo Business Innovation Center
    • Place of Presentation
      Sapporo, Japan
    • Year and Date
      20110908-10
  • [Presentation] New prevention strategy for inflammatory bowel disease using probiotic lactic acid bacteria and/or Bifidobacterium with specific adhesion to human intestine2011

    • Author(s)
      Saito, T., R. Yukishita, M. Watanabe, H. Kitazawa, Y. Kawai, A. Horii, F. Ikezawa, A. Yamamura, H. Ogawa, K. Miura, C. Shibata, I. Sasaki
    • Organizer
      10th Symposium on Lactic Acid Bacteria
    • Place of Presentation
      Egmond aan Zee, Netherland
    • Year and Date
      20110828-0901
  • [Presentation] Lactobacillus mucosae由来ABCトランスポータータンパク質(Lam29)のヒト大腸粘液中の新規受容体様分子の同定2011

    • Author(s)
      渡辺真通、江口慧、鶴見卓也、川井泰、北澤春樹、齋藤忠夫、山村明寛、生澤史江、三浦康、小川仁、佐々木巌、堀井明、木村勝紀、竹友直生
    • Organizer
      日本乳酸菌学会2011年度大会、関西大学
    • Place of Presentation
      大阪
    • Year and Date
      20110711-12
  • [Presentation] New Prevention Strategy for Inflammatory Bowel Disease Using Probiotic Lactic Acid Bacteria and/or Bifidobacterium Yogurt. Product Development Division-Food Microbiology Division. Application of Functional Microorganisms inFermented Food. "Food Biosciences: Pomises & Challenges in the Future"2011

    • Author(s)
      Saito, T
    • Organizer
      2011 Annual Meeting of Korean Society of Food Science and Technology (KoSFoST)
    • Place of Presentation
      EXCO, Daegu, Korea
    • Year and Date
      20110608-10

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Published: 2014-08-29  

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