2012 Fiscal Year Final Research Report
Mechanism of Listeria virulence factor-mediated regulation of innate immune response via swapping of virulence genes among Listeria species
| Project/Area Number |
22390082
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | 細菌 / リステリア / 病原因子 / 免疫応答 |
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| Research Abstract |
In the present study the molecular mechanism by which Listeria virulence factors regulate host innate immune response was investigated by swapping of corresponding virulence genes among Listeria species. Peritoneal exudate macrophages were infected with various Lm mutants, and cytokine production and the inflammasome activation was monitored. Results showed that AIM2 serves as an intracellular sensor of Lm to induce inflammasome and caspase-1 activation. ASC also plays an essential role incaspase-1 activation after entry of Lm into the cytoplasm. Furthermore, it was clearly shown that the Thr233 of LLO is an important residue to induce caspase-1 activation
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[Journal Article] Del Carmen Sasiain M. Two genetically-related multidrug-resistant Mycobacterium tuberculosis strains induce divergent outcomes of infection in two human macrophage models2013
Author(s)
Yokobori N, Lopez B, Geffner L, Sabio Y Garcia C, Schierloh P, Barrera L, de la Barrera S, Sakai S, Kawamura I, Mitsuyama M, Ritacco V
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Journal Title
Infect Genet Evol.
Volume: 16
Pages: 151-156
DOI
Peer Reviewed
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[Journal Article] Critical roles of ASC inflammasomes in caspase-1 activation and host innate resistance to Streptococcus pneumoniaeinfection.2011
Author(s)
Fang, K. Tsuchiya, I. Kawamura, Y. Shen, H. Hara, S. Sakai, T. Yamamoto, T. Fernandes-Alnemri, R. Yang, E. Hernandez-Cuellar, S. R. Dewamitta, Y. Xu, H. Qu, E. S. Alnemri, and M. Mitsuyama
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J. Immunol
Volume: 187
Pages: 4890-4899
DOI
Peer Reviewed
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[Journal Article] Expression of the Mycobacterium tuberculosis PPE37 protein in Mycobacterium smegmatis induces low tumour necrosis factor alpha and interleukin 6 production in murine macrophages.2011
Author(s)
S. Daim, T. Nomura, I. Kawamura, H. Hara, K. Tsuchiya, T. Kurenuma, Y. Shen, T. Yamamoto, H. Qu, S. Sakai, Y. Xu, and M. Mitsuyama.
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Journal Title
J. Med. Microbiol
Volume: 60
Pages: 582-591
DOI
Peer Reviewed
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[Journal Article] Toll-like receptor 2- and MyD88-dependent phosphatidylinositol 3-kinase and Rac1 activation facilitates the phagocytosis of Listeria monocytogenes by murine macrophages. .2010
Author(s)
Y. Xhen, I. Kawamura, T. Nomura, K. Tsuchiya, H. Hara, S. R. Dewamitta, S. Sakai, H. Qu, S. Daim, T. Yamamoto, and M. Mitsuyama.
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Journal Title
Infect. Immun.
Volume: 78
Pages: 2857-2867
DOI
Peer Reviewed
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[Journal Article] Listeriolysin O-dependent bacterial entry into the cytoplasm is required for calpain activation and interleukin-1 alpha secretion in macrophages infected with Listeria monocytogenes.2010
Author(s)
S.R. Dewamitta T. Nomura, I. Kawamura, H. Hara, K. Tsuchiya, T. Kurenuma, Y. Shen, S. Daim, T. Yamamoto, H. Qu, S. Sakai, Y. Xu, and M. Mitsuyama.
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Journal Title
Infect. Immun.
Volume: 78
Pages: 1884-1894
DOI
Peer Reviewed
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