2012 Fiscal Year Final Research Report
Clarification of mechanisms how H. pylori virulence factor OipA produces cytokines
| Project/Area Number |
22390085
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bacteriology (including Mycology)
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| Research Institution | Oita University |
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Principal Investigator |
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| Research Collaborator |
GRAHAM David Y ベイラー医科大学, 消化器内科, 教授
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| Project Period (FY) |
2010 – 2012
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| Keywords | ヘリコバクター・ピロリ / 胃癌 / シグナル伝達 / 微生物 / 感染症 |
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| Research Abstract |
In this study, we tried to clarify the mechanisms how H. pylori virulence factor OipA induce cytokines from gastric epithelial cells. First, we successfully constructed oipA mutants with either of 3 regions which I hypothesized to relate to cytokine expression mutated (including complementation mutants). However all of 3 regions were not strongly involved in cytokine induction from gastric epithelial cells. In experiments using oipA mutants without the function of OipA, we found that OipA is involved in the activation of Paxillin and FoxO related cytokine-induction signaling. In animal models, oipA mutants did not infect mice, suggesting that OipA is involved in the establishment of infection to gastric mucosa.
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