Establishment of the purification and culture methods of human adipose-derived stem cells as an autogenous graft to ischemic brain tissue

2012 Fiscal Year Final Research Report

• Project/Area Number: 22390274
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Cerebral neurosurgery
• Research Institution: Gifu University
• Principal Investigator: YOSHIMURA Shinichi 岐阜大学, 大学院・医学系研究科, 准教授 (40240353)
• Co-Investigator(Kenkyū-buntansha): IWAMA Toru 岐阜大学, 大学院・医学系研究科, 教授 (20303498) ; YANO Hirohito 岐阜大学, 大学院・医学系研究科, 准教授 (00332685) ; ENOMOTO Yukiko 岐阜大学, 大学院・医学系研究科, 助教 (20377659) ; OHE Naoyuki 岐阜大学, 医学部附属病院, 講師 (60362159) ; SOEDA Akio 岐阜大学, 医学部附属病院, 助教 (20444276)
• Co-Investigator(Renkei-kenkyūsha): EGASHIRA Yusuke 岐阜大学, 医学部附属病院, 助教 (50547677) ; TANAKA Yoshitaka 岐阜大学, 医学部附属病院, 助教 (00402199) ; IKEGAME Yuka 岐阜大学, 大学院・医学系研究科, 助教 (50402201)
• Project Period (FY): 2010 – 2012
• Keywords: 脂肪由来幹細胞 / 脳虚血モデル / ヒト脂肪細胞 / 移植 / 培養

Research Abstract

In the present study, we tried to establish the methods of purification of human adipose-derived stem cells, and investigated to elucidate the neuroprotective effects and mechanisms of ADSCs againstacute cerebral ischemia. Administration of ADSCs had neuroprotective effects against experimental ischemic stroke and its therapeutic effects were mainly owing to neuroprotective and anti-inflammatory mechanisms of its secreting factors.

Research Products

• [Journal Article] A Rho kinase (ROCK) inhibitor fasudil prevents matrix metalloproteinase-9-related hemorrhagic transformation in mice treated with tissue plasminogen activator. (2012)
  • Author(s): Ishiguro M, Kawasaki K, Suzuki Y, Ishizuka F, Mishiro K, Egashira Y, Ikegaki I, Tsuruma K, Shimazawa M, Yoshimura S, Iwama T, Hara H
  • Journal Title: Neurosience Volume: 220 Pages: 302-312
  • Peer Reviewed
• [Journal Article] The conditioned medium of murine and human adipose-derived stem cells exerts neuroprotective effects against experimental stroke model (2012)
  • Author(s): Egashira Y, Sugitan S, Suzuk Y Mishiro K Tsuruma K Shimazaswa M, Yoshimura S, Iwama T, Hara H
  • Journal Title: Brain Res Volume: 1461 Pages: 87-95
  • Peer Reviewed
• [Journal Article] 2-Decenoic acid ethyl ester, a compound that elicits neurotrophin-like intracellular signals, facilitating functional recovery from cerebral nfarction in mice (2012)
  • Author(s): Tanaka Y, Fukumitsu H. Soumiya H Yoshimura S, Iwama T, Furukawa S
  • Journal Title: Int J Mol Sci Volume: 13 Pages: 4968-4981
  • Peer Reviewed
• [Journal Article] Pharmacological inhibition of TLR4-NOX4 signal protects against neuronal death in transient focal ischemia (2012)
  • Author(s): Suzuki Y Hattori K, Hamanaka J, Murase T, Egashira Y, Mishiro K, Ishiguro M, Tsuruma K Hirose Y, Tanaka H, Yoshimura S, Shimazawa M, Inagaki N, Nagasawa H Iwama T, Hara H
  • Journal Title: Sci Rep Volume: 2 Pages: 896
  • Peer Reviewed
• [Journal Article] Comparison of mesenchymal stem cells from adipose tissue and bone marrow for ischemic stroke therapy (2011)
  • Author(s): Ikegame Y, Yamashita K Hayashi SI, Mizuno H, Tawada M, You F, Yamada K, Tanaka Y, Egashira Y, Nakashima S, Yoshimura S, Iwama T
  • Journal Title: CYTOTHERAPY Volume: 13 Pages: 675-85
  • Peer Reviewed
• [Journal Article] Blockade of phosphodiesterase-III protects against oxygen-glucose deprivation in endothelial cells by upregulation of VE-cadherin (2011)
  • Author(s): Ishiguro M, Suzuki Y, Mishiro K, Kakino M, Tsuruma K, Shimazawa M, Yoshimura S Iwama T, Hara H
  • Journal Title: Curr Neurovasc Res Volume: 8 Pages: 86-94
  • Peer Reviewed
• [Journal Article] cAMP regulates ADP-induced HSP27 phosphorylation in human platelets (2011)
  • Author(s): Enomoto Y, Adach S, Doi T, Natsume H Kato K, Matsushima-Nishiwaki R Akamatsu S, Tokuda H, Yoshimura S, Otsuka T, Kozawa O, Iwama T
  • Journal Title: Int J Mol Med Volume: 27 Pages: 695-700
  • Peer Reviewed
• [Journal Article] Comparison of mesenchymal stem cells from adipose tissue and bone marrow for ischemic stroke therapy (2011)
  • Author(s): Ikegame Y, Yamashita K, Hayashi SI, Mizuno H Tawada M, You F, Yamada K, Tanaka Y, Egashira Y, Nakashima S, Yoshimura SI, Iwama T
  • Journal Title: Cytotherapy Volume: 13 Pages: 675-685
  • Peer Reviewed
• [Journal Article] Phosphodiesterase-III inhibitor prevents hemorrhagic transformation induced by focal cerebral ischemia in mice treated with tPA (2010)
  • Author(s): Ishiguro M, Mishiro K, Fujiwara Y, Chen H, Izuta H, Tsuruma K, Shimazawa M, Yoshimura S, Satoh M, Iwama T, Hara H
  • Journal Title: PLoS One Volume: 5(12) Pages: e15178
  • Peer Reviewed
• [Journal Article] Neutrophil elastase inhibitor prevents ischemic brain damage via reduction of vasogenic edema (2010)
  • Author(s): Ikegame Y, Yamashita K, Hayashi S, Yoshimura S, Nakashima S, Iwama T
  • Journal Title: Hypertens Res Volume: 33 Pages: 703-7
  • Peer Reviewed
• [Journal Article] The Growth Factor Progranulin Attenuates Neuronal Injury Induced by Cerebral Ischemia-Reperfusion Through the Suppression of Neutrophil Recruitment
  • Author(s): Egashira Y, Suzuki Y, Azuma Y, Mishiro K, Sugitani S, Tsuruma K, Shimazawa M, Yoshimura S, Kashimata M, Iwama T, Hara H
  • Journal Title: J Neuroinflammation
  • Peer Reviewed
• [Presentation] The Growth Factor Progranulin Suppresses Inflammatory Reactions and Attenuates Neuronal Injury Induced by Focal Cerebral Ischemia/Reperfusion in Mice (2013)
  • Author(s): Egashira Y,Sugitani S, Suzuki Y, Mishiro K, Tsuruma K, Shimazawa M, Yoshimura S,Iwama T, Hara H
  • Organizer: International Stroke Conference.Honolulu
  • Place of Presentation: USA
  • Year and Date: 2013-02-06
• [Presentation] マウス脳虚血モデルに対する成長因子プログラニュリンの保護作用 (2012)
  • Author(s): 江頭裕介, 杉谷 創, 鈴木悠起也, 鶴間一寛, 嶋澤雅光, 吉村紳一, 岩間 亨, 原 英彰
  • Organizer: 第24回日本脳循環代謝学会総会
  • Place of Presentation: 広島
  • Year and Date: 2012-11-08
• [Presentation] マウス脳梗塞モデルに対する脂肪組織由来幹細胞培養上清の保護作用 (2012)
  • Author(s): 江頭裕介, 杉谷 創, 鶴間一寛, 嶋澤雅光, 吉村紳一, 岩間 亨, 原 英彰
  • Organizer: 第71回日本脳神経外科学会学術総会
  • Place of Presentation: 大阪
  • Year and Date: 2012-10-19
• [Presentation] マウス脳虚血モデルに対する脂肪組織由来幹細胞培養上清の保護作用 (2011)
  • Author(s): 江頭裕介, 杉谷 創, 鈴木悠希也, 鶴間一寛, 嶋澤雅光 吉村紳一, 岩間 亨, 原 英彰
  • Organizer: 第23回日本脳循環代謝学会総会
  • Place of Presentation: 東京
  • Year and Date: 2011-11-05
• [Presentation] マウス中大脳動脈虚血再灌流モデルに対するtPAとシロスタゾールの併用療法の検討 (2010)
  • Author(s): 石黒光紀, 三代圭祐, 藤原泰之, 陳 華岳, 伊豆田洋志, 鶴間一寛, 嶋澤雅光, 吉村紳一. 佐藤雅彦, 岩間 亨, 原 英彰
  • Organizer: 第22回日本脳循環代謝学会総会
  • Place of Presentation: 大阪
  • Year and Date: 2010-11-27
• [Presentation] マウス中大脳動脈虚血再灌流モデルに対するrt-PAとシロスタゾールの併用療法の検討 (2010)
  • Author(s): 石黒光紀, 嶋澤雅光, 吉村紳一, 岩間 亨, 原 英彰
  • Organizer: 第35回日本脳卒中学会総会
  • Place of Presentation: 盛岡
  • Year and Date: 2010-04-16
• [Remarks]
  • URL: http://www.med.gifu-u.ac.jp/neurosurgery/