2013 Fiscal Year Final Research Report
Search for novel biomarkers and therapeutic targets of malignant bone and soft tissue tumors/sarcomas using the methods of global expression analysis
| Project/Area Number |
22390296
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | National Center of Neurology and Psychiatry |
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Principal Investigator |
KAWAI AKIRA 独立行政法人国立がん研究センター, 中央病院, 医長 (90252965)
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| Co-Investigator(Kenkyū-buntansha) |
KONDO Tadashi 独立行政法人国立がん研究センター研究所, 創薬プロテオーム研究分野, 分野長 (30284061)
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| Co-Investigator(Renkei-kenkyūsha) |
ICHIKAWA Hitoshi 独立行政法人早期, 探索臨床研究センター, ユニット長 (30201924)
OCHIYA Takahiro 独立行政法人国立がん研究センター研究所, 分子細胞治療研究分野, 分野長 (60192530)
HOSONO Ako 国立がん研究センター, 東病院, 医長 (00602947)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 骨軟部腫瘍 / 肉腫 / 骨肉腫 / バイオマーカー / 遺伝子 / microRNA / タンパク質 / がん幹細胞 |
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| Research Abstract |
In the current study, we tried to identify novel factors determining the biological characteristics (chemosensitivity, invasiveness and development of metastasis) of malignant bone and soft tissue tumors (sarcomas). For osteosarcoma, we found peroxiredoxin-2 as a novel chemotherapy responsiveness marker and argininosuccinate synthetase and microRNA-143 as pulmonary metastasis predictive markers.
Silencing of microRNA-133a with locked nucleic acid (LNA) reduced cell invasion of highly malignant CD133 (high) population of osteosarcoma cells, and systemic administration of the LNA along with chemotherapy suppressed lung metastasis and prolonged the survival of osteosarcoma-bearing mice.
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