2012 Fiscal Year Final Research Report
Clinical application of diagnosis method of bovine major histocompatibility complex genes associated with resistanceand susceptibility to bovine leukemia virus-induced lymphomain East Asia
| Project/Area Number |
22405040
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Single-year Grants |
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| Section | 海外学術 |
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| Research Field |
Applied veterinary science
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
AIDA Yoko 独立行政法人理化学研究所, 分子ウイルス学特別研究ユニット, ユニットリーダー (50182994)
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| Co-Investigator(Renkei-kenkyūsha) |
ONUMA Misao 北海道大学, (連合)獣医学研究科, 名誉教授 (70109510)
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| Project Period (FY) |
2010 – 2012
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| Keywords | BoLA クラスIIDRB3 / PCR-Sequence based typing(SBT)法 / BLV,BLV nested PCR / フィリピン / ホルスタイン / ブラーマン / Native cattle |
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| Research Abstract |
We previously demonstrated that bovine leukocyte antigens (BoLA)-DRB3 alleles associated with resistance or susceptibility to development of bovine leukemia virus (BLV)-induced B lymphoma and analyzed distribution of these alleles in Japan and South-America. However, until now, none of the South-east Asian breed had been characterized for BoLA-DRB3 genes by PCR-sequence based typing (SBT). Hence, we here sequenced exon 2 of the BoLA class II DRB3 gene from 981 cattle including Holstein, Sahiwal, Brahman, Philippine Native and its cross breed, corrected from five islands of Philippine using modified SBT method which is suitable for Philippine cattle. In addition, we detected BLV by a new BLV-CoCoMo-qPCR. We identified the 75 previously reported alleles and 6 novel alleles. Interestingly, the number found in each breed is extent high level, indicating that that Asian breeds reveal extent high level of polymorphism for BoLA-DRB3 distributions. Our result also showed BLV-infected cattle with the susceptibility-associated allele was significantly lower than that in Japan.
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[Journal Article] Ohashi K., Enhanced expression of LAG-3 on lymphocyte subpopulations from persistently lymphocytotic cattle infected with bovine leukemia virus2013
Author(s)
Konnai, S., Suzuki, S., Shirai, T., Ikebuchi, R., Okagawa, T., Sunden., Y, Mingala C.N., Onuma, M., Murata, S
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Journal Title
Comp. Immunol. Microbiol. Infect. Dis
Volume: 36
Pages: 63-69
Peer Reviewed
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[Journal Article] Increased bovine Tim-3 and its ligand expressions during bovine leukemia virus infection2012
Author(s)
Okagawa, T., Konnai, S., Ikebuchi, R., Suzuki, S., Shirai, T., Sunden, Y., Onuma M., Murata, S., Ohashi, K
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Journal Title
Vet. Res
Volume: 43
Pages: 45
Peer Reviewed
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[Journal Article] Increase of cells expressing PD-L1 in bovine leukemia virus infection and enhancement of anti-viral immune responses in vitro via PD-L1 blockade2011
Author(s)
Ikebuchi, R., Konnai, S., Shirai, T., Sunden, Y., Murata, S., Onuma M., Ohashi, K
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Journal Title
Vet. Res
Volume: 42
Pages: 103
Peer Reviewed
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[Journal Article] Molecular cloning of bovine lymphocyte activation gene-3 and its expression characteristics in bovine leukemia virus-infected cattle. Vet. Immunol2011
Author(s)
Shirai, T., Konnai, S., Ikebuchi, R., Okagawa, T., Suzuki, S., Sunden, Y., Onuma, M., Murata, S., Ohashi, K
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Journal Title
Immunopathol
Volume: 44
Pages: 462-467
Peer Reviewed
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[Presentation] Th epitope mapping of BLV envelope protein for disease susceptibility cattle determined by major hisutocompatibility complex class II DRB3 allele2011
Author(s)
Takeshima S-n., Hagiwara K., Matsumoto Y., Kim J., Miyasaka T., Jimba M., Hibishima T., Ohmori T., Nunoya T., Matoba K., Aida Y
Organizer
The XXV Symposium of the International Association for Comparative Researchi on Leukemia and Related Diseases
Year and Date
20110915-17
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[Presentation] Variation of cattle major histocompatibility complex(BOLA)DRB3 allele frequencies within different farm,breed and coutries in South America2010
Author(s)
竹嶋伸之輔、松本有生、Arainga M., 金智潤、宮坂卓、薛光愛、 Veronica Gabriela de la Barra Diaz, Giovambattista G., Enrique PofcherJ., Hermelinda,Rivera-Geronimo, 斉藤英樹,Thomas Acosta J.,印牧美佐生、 Manuel Ortiz L., Jorge Oltra,小沼操、 間陽子
Organizer
32nd Conference of the International Society for Animal Genetics
Place of Presentation
スコットランド
Year and Date
20100726-30
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