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2012 Fiscal Year Final Research Report

Development of an assay to evaluate the reactivity at chemicals contaminated in the atmosphere, South California

Research Project

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Project/Area Number 22406004
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypeSingle-year Grants
Section海外学術
Research Field Environmental pharmacy
Research InstitutionUniversity of Tsukuba

Principal Investigator

KUMAGAI Yoshito  筑波大学, 医学医療系, 教授 (00250100)

Co-Investigator(Kenkyū-buntansha) SHINKAI Yasuhiro  筑波大学, 医学医療系, 助教 (10454240)
Project Period (FY) 2010 – 2012
Keywords大気中揮発物質 / 親電子物質 / Keap1/Nrf2 / グルタチオン
Research Abstract

We describe here results of studies investigating the actions of the vapor phase of ambient air samples collected in the midtown area of Los Angeles on human bronchial epithelial BEAS-2B cells using DNA microarray analysis. Among 26 genes whose expression increased 4 fold or more, 4 genes were associated with detoxifying genes regulated by the transcription factor Nrf2. Consistent with these results, the vapor samples activate the Nrf2-ARE pathway, resulting in up-regulation of heme oxygenase-1 (HO-1), glutamate cysteine ligase modifier subunit, and cystine transporter (xCT) mRNA and proteins. No appreciable increases in pro-inflammatory genes were observed. These results suggest that ambient vapor samples activate the Nrf2-ARE pathway but not an inflammatory response.

  • Research Products

    (16 results)

All 2013 2012 2011 2010 Other

All Journal Article (9 results) (of which Peer Reviewed: 9 results) Presentation (5 results) Book (1 results) Remarks (1 results)

  • [Journal Article] Interaction of 9,10-phenanthraquinone with dithiol causes oxidative modification of Cu,Znsuperoxide dismutase (SOD) through redox cycling2013

    • Author(s)
      Koizumi R, Taguchi K, Hisamori M, Kumagai Y.
    • Journal Title

      Journal of Toxicological Sciences

      Volume: 38 Pages: 317-324

    • DOI

      DOI:org/10.2131/jts.38.317

    • Peer Reviewed
  • [Journal Article] A method for detecting covalent modification of sensor proteins associated with 1,4-naphthoquinone-inducedactivation of electrophilic signal transduction pathways.2012

    • Author(s)
      Hirose R, Miura T, Sha R, Shinkai Y, Tanaka-Kagawa T, Kumagai Y.
    • Journal Title

      Journal of Toxicological Sciences

      Volume: 37 Pages: 891-898

    • DOI

      DOI:10.2131/jts.37.891

    • Peer Reviewed
  • [Journal Article] The chemical biology of naphthoquinones and its environmental implications2012

    • Author(s)
      Kumagai Y, Shinkai Y, Miura T, Cho AK.
    • Journal Title

      Annual Review of Pharmacology and Toxicology

      Volume: 52 Pages: 221-247

    • DOI

      DOI:10.1146/annurev-pharmtox-010611-134517

    • Peer Reviewed
  • [Journal Article] Peroxiredoxin 6 is a molecular target for 1,2-naphthoquinone, an atmospheric electrophile, in human pulmonary epithelial A549 cells2011

    • Author(s)
      Takayama N, Iwamoto N, Sumi D, Shinkai Y, Tanaka-Kagawa T, Jinno H, Kumagai Y.
    • Journal Title

      Journal of Toxicological Sciences

      Volume: 36 Pages: 817-821

    • DOI

      DOI:10.2131/jts.36.817

    • Peer Reviewed
  • [Journal Article] Initial response and cellular protection through the Keap1/Nrf2 system during exposure of primary mouse hepatocytes to 1,2-naphthoquinone2011

    • Author(s)
      Miura T, Shinkai Y, Jiang HY, Iwamoto N, Sumi D, Taguchi K, Yamamoto M, Jinno H, Tanaka-Kagawa T, Cho AK, Kumagai Y.
    • Journal Title

      Chemical Research in Toxicology

      Volume: 24 Pages: 559-567

    • DOI

      DOI:10.1021/tx100427p

    • Peer Reviewed
  • [Journal Article] Diesel exhaust particles induce a Th2 phenotype in mouse naive mononuclear cells in vitro2010

    • Author(s)
      Inoue K, Koike E, Endo A, Sumi D, Kumagai Y, Hayakawa K, Kiyono M, Tanaka M, Takano H.
    • Journal Title

      Experimental and Therapeutic Medicine

      Volume: 1 Pages: 761-767

    • DOI

      DOI:10.3892/etm.2010.129

    • Peer Reviewed
  • [Journal Article] Redox and electrophilic properties ofvapor- and particle-phase components of ambient aerosols2010

    • Author(s)
      Eiguren-Fernandez A, Shinyashiki M, Schmitz DA, Distefano E, Hinds W, Kumagai Y, Cho AK, Froines JR
    • Journal Title

      Environmental Research

      Volume: 110 Pages: 207-212

    • DOI

      DOI:10.1016/j.envres.2010.01.009

    • Peer Reviewed
  • [Journal Article] Biochemical and cellular effects of electrophiles present in ambient air samples2010

    • Author(s)
      Iwamoto N, Nishiyama A, Eiguren-Fernandez A, Hinds W, Kumagai Y, Froines JR, Cho AK, Shinyashiki M
    • Journal Title

      Atmospheric Environment

      Volume: 44 Pages: 1483-1489

    • DOI

      DOI:10.1016/j.atmosenv.2010.01.045

    • Peer Reviewed
  • [Journal Article] Involvement of sensory nerves and TRPV1 receptors in the rat airway inflammatory response to two environment pollutants: diesel exhaust particles (DEP) and 1,2-naphthoquinone (1,2-NQ)2010

    • Author(s)
      Teles AM, Kumagai Y, Brain SD, Teixeira SA, Varriano AA, Barreto MA, de Lima WT, Antunes E, Muscara MN, Costa SK.
    • Journal Title

      Archives of Toxicology

      Volume: 84 Pages: 109-117

    • DOI

      DOI:10.1007/s00204-009-0427-x.

    • Peer Reviewed
  • [Presentation] Activation of Heat Shock Response during exposure of human epithelial A431 cells to an environmental electrophile, 1,4-naphthoquinone2013

    • Author(s)
      Sha R, Shinkai Y, Kumagai Y.
    • Organizer
      第12回分子予防環境医学研究会
    • Place of Presentation
      つくば
    • Year and Date
      2013-02-02
  • [Presentation] 環境中親電子物質によるタンパク質の化学修飾とそれに対する応答戦略2012

    • Author(s)
      熊谷嘉人
    • Organizer
      第39回日本毒性学会学術年会シンポジウム
    • Place of Presentation
      仙台
    • Year and Date
      2012-07-19
  • [Presentation] Invasion of environmental electrophiles into cells, resulting in a modulation of cellular signaltransduction pathways2012

    • Author(s)
      Kumagai Y
    • Organizer
      UK-Japan Research Symposium. Molecular Mechanisms of Stress Response in Disease
    • Place of Presentation
      つくば
    • Year and Date
      2012-04-07
  • [Presentation] 大気中親電子物質:タンパク質の化学修飾を介した有害反応とそれに対する生体側の防御戦略2011

    • Author(s)
      熊谷嘉人
    • Organizer
      日本自動車研究所講演会
    • Place of Presentation
      東京
    • Year and Date
      2011-11-25
  • [Presentation] 環境中親電子物質に対する生体応答およびリスク軽減戦略2011

    • Author(s)
      熊谷嘉人.
    • Organizer
      第4回エコファーマシンポジウム
    • Place of Presentation
      熊本
    • Year and Date
      2011-02-08
  • [Book] 改定版分子予防環境医学2010

    • Author(s)
      熊谷嘉人
    • Total Pages
      576-583
    • Publisher
      本の泉社
  • [Remarks]

    • URL

      http://www.md.tsukuba.ac.jp/environmental_medicine/index.html

URL: 

Published: 2014-08-29  

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