2012 Fiscal Year Final Research Report
Generation of multi-step, site-specific recombination system in vivo
| Project/Area Number |
22500384
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Laboratory animal science
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
ICHISE Taeko 東京大学, 医科学研究所, 助教 (00396863)
YOSHIDA Nobuaki 東京大学, 医科学研究所, 教授 (10250341)
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| Project Period (FY) |
2010 – 2012
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| Keywords | Cre/loxP / FLP/FRT / Dre/rox / 部位特異的組換え酵素 / マウス |
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| Research Abstract |
We identified the transgene integration site of the CGH4 transgenic mice that conditionally express H-Ras and EGFP in a variety of tissues in a Cre/loxP recombination-dependent manner (Ichise et al., 2010), generated new mouse lines using targeting vectors for the locus, and found that the locus is suitable for site-spesific recomibinase-mediated, binary expression of transgenes in an endothelial cell lineage. Targeted transgenesis and binary expression system using the locus in combination with the Rosa26 locus will be valuable for developing multi-step, site-specific recombination system in vivo.
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Research Products
(13 results)
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[Journal Article] Y.Platelet activation receptor CLEC-2 regulates blood/lymphatic vessel separation by inhibiting proliferation, migration, and tube formation of lymphatic endothelial cells.2012
Author(s)
Osada, M., Inoue, O., Ding, G., Shirai, T., Ichise, H., Hirayama, K., Takano, K., Yatomi, Y., Hirashima, M., Fujii, H., Suzuki-Inoue, K., Ozaki
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Journal Title
The Journal of biological chemistry.
Volume: 287
Pages: 22241-22252
DOI
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[Journal Article] PTB Deficiency Causes the Loss of Adherens Junctions in the Dorsal Telencephalon and Leads to LethalHydrocephalus.2012
Author(s)
Shibasaki, T., Tokunaga, A., Sakamoto, R., Sagara, H., Noguchi, S., Sasaoka, T., Yoshida, N.
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Journal Title
Cerebral cortex.Epub ahead of print, Jun 15
DOI
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[Journal Article] N., Miki, H.Nucleoredoxin sustains Wnt/β-catenin signaling by retaining a pool of inactive Dishevelled protein.2010
Author(s)
Funato, Y., Terabayashi, T., Sakamoto, R., Okuzaki, D., Ichise, H., Nojima, H.,Yoshida
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Journal Title
Current biology.
Volume: 20
Pages: 1945-1952
DOI
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[Journal Article] Nucleoredoxin negatively regulates Toll-like receptor 4 signaling via recruitment of flightless-I to myeloid differentiation primary response gene (88).2010
Author(s)
Hayashi, T., Funato, Y., Terabayashi, T., Morinaka, A., Sakamoto, R., Ichise, H., Fukuda, H., Yoshida, N., Miki, H.
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Journal Title
The Journal of biological chemistry.
Volume: 285
Pages: 18586-18593
DOI
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