2012 Fiscal Year Final Research Report

Involvement of Polycomb group genes in cell line property of human ES and iPS cells

Research Project

Project/Area Number	22500425
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Biomedical engineering/Biological material science
Research Institution	Shinshu University
Principal Investigator	TOMOTSUNE Daihachiro 信州大学, 医学部, 助教 (80283802)
Co-Investigator(Renkei-kenkyūsha)	SASAKI Katsunori 信州大学, 医学部, 教授 (30170666) YUE Fengming 信州大学, 医学部, 助教 (20532865)
Research Collaborator	MOGI Akimi 信州大学, 医学系研究科, 大学院生
Project Period (FY)	2010 – 2012
Keywords	ヒト ES 細胞 / ヒトiPS 細胞 / ポリコーム
Research Abstract	In this study, we produced the spontaneous differentiation, and then analyzed global changes of gene expression during the spontaneous differentiation using DNA microarray. As results of the microarray analysis using four human pluripotent stem cell lines, we found that some genes were commonly up- or down-regulated during the collapse of undifferentiated state. Furthermore, we analyzed the role of polycomb group during the collapse of undifferentiated state by ChIP-seq and ChIP-QPCR.