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2012 Fiscal Year Final Research Report

Involvement of Polycomb group genes in cell line property of human ES and iPS cells

Research Project

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Project/Area Number 22500425
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Biomedical engineering/Biological material science
Research InstitutionShinshu University

Principal Investigator

TOMOTSUNE Daihachiro  信州大学, 医学部, 助教 (80283802)

Co-Investigator(Renkei-kenkyūsha) SASAKI Katsunori  信州大学, 医学部, 教授 (30170666)
YUE Fengming  信州大学, 医学部, 助教 (20532865)
Research Collaborator MOGI Akimi  信州大学, 医学系研究科, 大学院生
Project Period (FY) 2010 – 2012
Keywordsヒト ES 細胞 / ヒトiPS 細胞 / ポリコーム
Research Abstract

In this study, we produced the spontaneous differentiation, and then analyzed global changes of gene expression during the spontaneous differentiation using DNA microarray. As results of the microarray analysis using four human pluripotent stem cell lines, we found that some genes were commonly up- or down-regulated during the collapse of undifferentiated state. Furthermore, we analyzed the role of polycomb group during the collapse of undifferentiated state by ChIP-seq and ChIP-QPCR.

  • Research Products

    (2 results)

All 2013

All Journal Article (1 results) (of which Peer Reviewed: 1 results) Presentation (1 results)

  • [Journal Article] Fluid dynamic forces created by rotary orbital suspension culture promote cardiomyogenic differentiation of human embryonic stem cells.2013

    • Author(s)
      Akimi Mogi, Shunsuke Takei, Hisashi Shimizu, Haruki Miura, Daihachiro Tomotsune*, and Katsunori Sasaki
    • Journal Title

      J. Med. Biol. Eng

      Volume: (印刷中)

    • DOI

      DOI:;10.5405/jmbe.1356

    • Peer Reviewed
  • [Presentation] GLOBAL ANALYSIS OF STABILITY OF UNDIFFERENTIATED STATE IN HUMAN PLURIPOTENT STEM CELLS2013

    • Author(s)
      Daihachiro Tomotsune, Akimi Mogi, Fengming Yue, Susumu Yoshie, Shoko Masuda, Ayana Sakurai, Daiki Fujita, Mika Morisaki-Nagai, Sakiko Takizawa-Shirasawa, Tadayuki Yokoyama, Katsunori Sasaki
    • Organizer
      第11回国際幹細胞学会(ISSCR)
    • Place of Presentation
      ボストン(アメリカ合衆国)
    • Year and Date
      2013-06-12

URL: 

Published: 2014-08-29  

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