2012 Fiscal Year Final Research Report
Identification and validation of biomarker proteins based ontheir thiol redox states
| Project/Area Number |
22501035
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Tumor diagnosis
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| Research Institution | Tokyo Medical University |
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Principal Investigator |
KAWAKAMI Takao 東京医科大学, 医学部, 客員准教授 (40366117)
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| Co-Investigator(Renkei-kenkyūsha) |
YANO Hiroyuki 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所, ユニット長 (20355580)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 酸化還元 / プロテオーム / 化学修飾 / 質量分析 / 翻訳後修飾 / 配列データベース |
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| Research Abstract |
Cellular reduction-oxidation (redox) states are regulated mainly by the cysteine thiol groups in protein molecules. Using a fluorescent chemical probe specific with reductive thiol groups, we established reaction conditions for quantitative thiol modifications. Non-reduction/reduction two-dimensional electrophoresis is a promising choice towards comprehensive redox state analysis. Polyacrylamide gel strip backing with a plastic film enabled higher reproducibility of the first dimensional separation. In addition, we made a program of extracting peptide information containingselected post-translational modification(s) from public sequence databases.
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