Clinical Application of DNA Methylation Analysis Using Gastric Washes as an ESD Treatment Marker for Early Gastric Cancer

2012 Fiscal Year Final Research Report

• Project/Area Number: 22501038
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Tumor diagnosis
• Research Institution: St. Marianna University School of Medicine
• Principal Investigator: WATANABE Yoshiyuki 聖マリアンナ医科大学, 医学部, 講師 (90329243)
• Project Period (FY): 2010 – 2012
• Keywords: 胃がん / メチル化 / 胃洗浄廃液

Research Abstract

We have developed a new methodology for gastric cancer detection by DNA methylation in gastric washes. It is easy and useful for early detection of recurrence after endoscopic treatment in GCa patients. We now have on going prospective studies.

Research Products

• [Journal Article] Hypermethylation of Sox17 gene is useful as a molecular diagnostic application in early gastric cancer. (2012)
  • Author(s): Watanabe Y, Oishi Y, Yoshida Y, Sato Y, Hiraishi T, Oikawa R, Maehata T, Suzuki H, Toyota M, Niwa H, Suzuki M, Itoh F.
  • Journal Title: Tumour Biol Volume: 33(2) Pages: 383-93
• [Journal Article] Characterization of DNA hypermethylation in two cases of peritoneal mesothelioma. (2012)
  • Author(s): Watanabe Y, Hama R, Shinada K, Yamada Y, Ogata Y, Yoshida Y, TamuraT, Hiraishi T, Oikawa R, Sakurai J, Maehata T, Koizumi H, Itoh F.
  • Journal Title: Tumor Biol. Volume: 33(6) Pages: 2031-40
• [Journal Article] Frequent alteration of MLL3 frameshift mutations in microsatellite deficient colorectal cancer. (2011)
  • Author(s): Watanabe Y, Castoro RJ, Kim HS, North B, Oikawa R, Hiraishi T, Ahmed SS, Chung W, Cho MY, Toyota M, Itoh F, Estecio MR, Shen L, Jelinek J, Issa JP.
  • Journal Title: PLos One Volume: 6 Pages: e23320
• [Journal Article] Gastric wash-based molecular testing for antibiotic resistance in helicobacter pylori. (2011)
  • Author(s): Baba S, Oishi Y, Watanabe Y, Oikawa R, Morita R, Yoshida Y, Hiraishi T, Maehata T, Nagase Y, Fukuda Y, Nakazawa M, Ishigouoka S, Hattori N, Suzuki H, Toyota M, Niwa H, Suzuki M, Itoh F.
  • Journal Title: Digestion Volume: 84 Pages: 299-305
• [Journal Article] DNA methylation profiles of primary colorectal carcinoma and matched liver metastasis. (2011)
  • Author(s): Watanabe Y, Konishi K, Shen L, Guo Y, Castoro RJ, Kondo K, Chung W, Ahmed S, Jelinek J, Boumber YA, Estecio MR, Maegawa S, Kondo Y, Itoh F, Imawari M, Hamilton SR, Issa JP.
  • Journal Title: PLos One Volume: 6 Pages: e27889
• [Presentation] The BARHL2 Gene Hypermethylation is Useful as a Molecular Diagnostic Application in Early Stage of Gastric Cancer. (2012)
  • Author(s): Watanabe Y et al.
  • Organizer: American College of Gastroenterology Annual Meeting 2012.
  • Place of Presentation: Las Vegas NV. Unites States
  • Year and Date: 2012-10-20
• [Presentation] Gastric Wash-Based Sox17 Methylation is Useful as a Molecular Diagnostic Application in Early Stage of Gastric Cancer. (2012)
  • Author(s): Watanabe Y et al.
  • Organizer: Digestive Disease Week 2012.
  • Place of Presentation: San Diego CA United States
  • Year and Date: 2012-05-20
• [Presentation] The Sox17 Gene Hypermethylation is Useful as a Molecular Diagnostic Application in Early Stage of Gastric Cancer. (2012)
  • Author(s): Hama R, Watanabe Y et al.
  • Organizer: American Association for Cancer Research Annual Meeting 2012.
  • Place of Presentation: Chicago IL. United States
  • Year and Date: 2012-04-02
• [Patent(Industrial Property Rights)] 胃粘膜洗浄液を利用した疾患関連マーカー検出方法 (2007)
  • Inventor(s): 渡邊 嘉行、豊田 実、今井 浩三、篠村 恭久、伊東 文生、時野 隆至
  • Industrial Property Rights Holder: 学校法人聖マリアンナ医科大学、北海道公立大学法人札幌医科大学
  • Industrial Property Number: 特許 国際出願 PCT/JP2007/059953
  • Filing Date: 2007-05-15
  • Overseas