Analyses of the dynamics and influence of bioaerosols in the atmosphere of Toyama Prefecture, Japan

2012 Fiscal Year Final Research Report

• Project/Area Number: 22510026
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Environmental impact assessment/Environmental policy
• Research Institution: University of Toyama
• Principal Investigator: TANAKA Daisuke 富山大学, 大学院・理工学研究部(理学), 准教授 (40360804)
• Co-Investigator(Kenkyū-buntansha): NAKAMURA Shogo 富山大学, 大学院・理工学研究部(理学), 教授 (60134996)
• Project Period (FY): 2010 – 2012
• Keywords: 陸圏 / 水圏 / 大気圏影響評価

Research Abstract

Bacterial community structure was investigated by analyzing air samples collected almost weekly on the roof of the Faculty of Science, University of Toyama, Toyama Prefecture, Japan. PCR-DGGE （Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis) was used to analyze changes in the structure of the bacterial communities in the atmosphere. The DGGE profiles showed significant differences among sampling days. Overall, 106 bands were excised from the DGGE gels and subsequently sequenced. About 80% of sequences were affiliated with the phylaProteobacteria, Firmicutes, and Bacteroidetes, and the most common genus was Pseudomonas. We also investigated bacterial communities in Asian dust-containing snow layers on Mt. Tateyama. Interestingly, we detected Bacillus isolates with identical 16S rDNA sequences （AB500941) over 2 consecutive years, and these results might reflect the long-distance transportation of microorganisms from dust source regions.

Research Products

• [Journal Article] Bacterial communities in Asian dust-containing snow layers on Mt (2011)
  • Author(s): Tanaka, D., Tokuyama, Y., Terada, Y., Kunimochi, K., Mizumaki, C., Tamura, S., Wakabayashi, M., Aoki, K., Shimada, W., Tanaka, H., and Nakamura, S
  • Journal Title: Tateyama, Japan. Bulletin of Glaciological Research Volume: Vo l.29
  • Peer Reviewed
• [Presentation] Effects of Ultraviolet Radiation on Pigmented Airborne Bacterial Isolates from Toyama, Japan (2012)
  • Author(s): Yohei Mizuno, Daisuke Tanaka, Kota Kunimochi, Takuya Nakashima, Shogo Nakamura
  • Organizer: 第28回日本微生物生態学会大会
  • Place of Presentation: 豊橋技術科学大学(愛知)
  • Year and Date: 20120920-21
• [Presentation] 富山県の大気中における細菌群集の季節動態 (2011)
  • Author(s): 寺田陽平,徳山祐美,田中大祐,中村省吾
  • Organizer: 第27回日本微生物生態学会大会
  • Place of Presentation: 京都大学(京都)
  • Year and Date: 2011-10-08
• [Presentation] 立山における黄砂を含む積雪層中の細菌の分離・同定 (2011)
  • Author(s): 田中大祐,田村尚子,寺田陽平,青木一真,島田 亙,中村省吾
  • Organizer: 第5回大気バイオエアロゾルシンポジウム
  • Year and Date: 2011-03-30
• [Presentation] 黄砂を含む立山積雪中における細菌群集構造の解析 (2010)
  • Author(s): 田中大祐,徳山祐美,寺田陽平,国持孝太,青木一真,島田 亙,中村省吾
  • Organizer: 第27回エアロゾル科学・技術研究討論会
  • Place of Presentation: 名古屋大学(愛知)
  • Year and Date: 2010-08-04
• [Presentation] 高低差3000mから観るエアロゾルの季節変化 (2010)
  • Author(s): 青木一真,島田 亙,田中大祐,松 木 篤
  • Organizer: 第27回エアロゾル科学・技術研究討論会
  • Place of Presentation: 名古屋大学(愛知)
  • Year and Date: 2010-08-04