2012 Fiscal Year Final Research Report
Analyses of the dynamics and influence of bioaerosols in the atmosphere of Toyama Prefecture, Japan
Project/Area Number |
22510026
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Research Category |
Grant-in-Aid for Scientific Research (C)
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Allocation Type | Single-year Grants |
Section | 一般 |
Research Field |
Environmental impact assessment/Environmental policy
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Research Institution | University of Toyama |
Principal Investigator |
TANAKA Daisuke 富山大学, 大学院・理工学研究部(理学), 准教授 (40360804)
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Co-Investigator(Kenkyū-buntansha) |
NAKAMURA Shogo 富山大学, 大学院・理工学研究部(理学), 教授 (60134996)
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Project Period (FY) |
2010 – 2012
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Keywords | 陸圏 / 水圏 / 大気圏影響評価 |
Research Abstract |
Bacterial community structure was investigated by analyzing air samples collected almost weekly on the roof of the Faculty of Science, University of Toyama, Toyama Prefecture, Japan. PCR-DGGE (Polymerase Chain Reaction and Denaturing Gradient Gel Electrophoresis) was used to analyze changes in the structure of the bacterial communities in the atmosphere. The DGGE profiles showed significant differences among sampling days. Overall, 106 bands were excised from the DGGE gels and subsequently sequenced. About 80% of sequences were affiliated with the phylaProteobacteria, Firmicutes, and Bacteroidetes, and the most common genus was Pseudomonas. We also investigated bacterial communities in Asian dust-containing snow layers on Mt. Tateyama. Interestingly, we detected Bacillus isolates with identical 16S rDNA sequences (AB500941) over 2 consecutive years, and these results might reflect the long-distance transportation of microorganisms from dust source regions.
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Research Products
(6 results)
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[Journal Article] Bacterial communities in Asian dust-containing snow layers on Mt2011
Author(s)
Tanaka, D., Tokuyama, Y., Terada, Y., Kunimochi, K., Mizumaki, C., Tamura, S., Wakabayashi, M., Aoki, K., Shimada, W., Tanaka, H., and Nakamura, S
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Journal Title
Tateyama, Japan. Bulletin of Glaciological Research
Volume: Vo l.29
Peer Reviewed
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