2013 Fiscal Year Final Research Report
Controlling the higher order structure of single DNA molecule with attention to environmental stress
| Project/Area Number |
22510123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Nanomaterials/Nanobioscience
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| Research Institution | Ritsumeikan University |
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Principal Investigator |
YOSHIKAWA Yuko 立命館大学, 総合科学技術研究機構, 教授 (80291871)
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 1分子計測 / 環境応答 / 長鎖DNA / DNA損傷 / 蛍光顕微鏡 |
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| Research Abstract |
1. The protective effects of water-soluble derivatives of flavonoids on photo- and gamma ray-induced DNA double-strand breaks were evaluated in a quantitative manner. This observation demonstrated the dependence of the antioxidant potentials of these compounds on the source of irradiation. 2. It was found that tetrazolato-bridged dinuclear platinum(II) complexes induced the irreversible compaction of large genomic DNA, which was accompanied by a change in the secondary structure from the B to C form. 3.The appearance of the phase-segregated state in megabase-size DNA is markedly different from the all-or-none characteristics on the foldingtransition in a single sub-megabase-size DNA. 4. Novel stereoisomers of tetravalent polyamines were examined their ability to induce the compaction of single DNA molecules. It was found that Isomer (R,S-R,S) is more potent at inducing both DNA compaction and changes in the secondary structure.
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