2012 Fiscal Year Final Research Report
Proteomic analysis of bacterial translation by iso-electric point unlimited RFHR 2D PAGE
| Project/Area Number |
22510210
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genome biology
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| Research Institution | 株式会社吉田生物研究所 (2012)
Yoshida Biological Laboratry, Bio-informatics section (2010-2011) |
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Principal Investigator |
WADA Akira 株式会社吉田生物研究所, バイオ情報研究部門, 部門長 (80025387)
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| Co-Investigator(Kenkyū-buntansha) |
UETA Masami 株式会社吉田生物研究所, バイオ情報研究部, 研究員 (30512511)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 翻訳 / RFHR法 / プロテオミクス / リボソーム / 100Sリボソーム / 源核生物 |
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| Research Abstract |
Quantitative and separating ability of RFHR 2D PAGE was improved, and it was applied to proteomics of bacterial translation. In 15 bacterial strains proteins forming 100S ribosome(70S dimer) were determined. It is RMF homolog in proteobacteria γgroup, and long HPF homolog in other bacteria. Therefore two distinct 100S ribosomes exist in bacteria.
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Research Products
(31 results)
