NAD-related Metabolism and its Physiological Significance

2012 Fiscal Year Final Research Report

• Project/Area Number: 22510226
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Living organism molecular science
• Research Institution: Ochanomizu University
• Principal Investigator: ASHIHARA Hiroshi お茶の水女子大学, 名誉教授 (00017211)
• Project Period (FY): 2010 – 2012
• Keywords: 植物 / 生化学 / 代謝経路 / 生合成 / 分解 / 酵素 / 細胞組織

Research Abstract

The metabolism of pyridine nucleotides and related secondary metabolites in higher plants were investigated. A plant specific seven-membered pyridine nucleotide cycle and two bypass enzymes were discovered during the course of the study. Nicotinic acid glucoside formation was restricted to ferns and selected orders of angiosperms, whereas other plants synthesized trigonelline. The pyridine ring of nicotinic acid glucoside was degraded by a novel pathway which involved glutaric acid as anintermediate. The physiological function of pyridine conjugates formation is discussed.

Research Products

• [Journal Article] Pyridine metabolism in tea plants: Salvage, conjugate formation and catabolism (2012)
  • Author(s): Ashihara, H. and Deng, W
  • Journal Title: Journal of Plant Research Volume: Vol.125 Pages: 781-791
  • DOI: doi:10.1007/s10265-012-0490-x
• [Journal Article] Metabolic fate of 14C-labelled nicotinamide and adenine ingerminating propagules of the mangrove Bruguiera gymnorrhiza (2012)
  • Author(s): Yin, Y., Watanabe, S. and Ashihara, H
  • Journal Title: Zeitschrift Fur Naturforschung - Section C Journalof Biosciences Volume: Vol.66 Pages: 319-326
• [Journal Article] Comparison of the formation of nicotinic acid conjugates in leaves of different plant species (2012)
  • Author(s): Ashihara, H., Yin, Y., Katahira, R., Watanabe, S., Mimura, T. and Sasamoto, H
  • Journal Title: Plant Physiology and Biochemistry Volume: Vol. 60 Pages: 190-195
  • DOI: doi:10.1016/j.plaphy.2012.08.007
• [Journal Article] Pyridine metabolism and trigonellinesynthesis in leaves of the mangrove legume trees Derris indica (Millettia pinnata) and Caesalpinia crista (2011)
  • Author(s): Yin, Y.; Sasamoto, H. and Ashihara, H
  • Journal Title: Natural Product Communications Volume: Vol. 6 Pages: 1835-1838
• [Journal Article] Trigonelline biosynthesis and the pyridine nucleotide cycle in Coffea arabica fruits: Metabolic fate of [carboxyl-14C] nicotinic acid riboside (2011)
  • Author(s): Ashihara, H., Deng, W.and Nagai, C
  • Journal Title: Phytochemistry Letters Volume: Vol. 4 Pages: 235-239
  • DOI: doi:10.1016/j.phytol.2011.04.005
• [Journal Article] Nicotinamide metabolism inferns:Formation of nicotinic acid glucoside (2011)
  • Author(s): Ashihara, H., Yin, Y. and Watanabe, S
  • Journal Title: Plant Physiology and Biochemistry Volume: Vol. 49 Pages: 275-279
  • DOI: doi:10.1016/j.plaphy.2010.12.008
• [Journal Article] Pyridine salvage and nicotinic acid conjugate synthesis in leaves of mangrove species (2010)
  • Author(s): Ashihara, H., Yin, Y., Deng, W. andWatanabe, S
  • Journal Title: Phytochemistry Volume: Vol.71 Pages: 47-53
  • DOI: doi:10.1016/j.phytochem.2009.09.033
• [Book] Plant Metabolism and Biotechnology (2011)
  • Author(s): Ashihara, H., Crozier, A. and Komamine, A. Wiley
  • Total Pages: 1-422