2012 Fiscal Year Final Research Report
Exploration of chemical probes for investigation of oxidative metabolism of auxins
| Project/Area Number |
22510228
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Living organism molecular science
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| Research Institution | Kyoto University |
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Principal Investigator |
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| Project Period (FY) |
2010 – 2012
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| Keywords | オーキシン / 異化代謝 / 質量分析 / スクリーニング / 酸化酵素 |
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| Research Abstract |
Indole-3-acetic acid is a class of plant hormones, which is responsible for plant growth regulation. The concentration of IAA is strictly controlled by metabolism and transport. In IAA metabolism, oxidation plays an important role, but its mechanisms are not fully understood. In this study, we developed a screening system for inhibitors of IAA oxidation. As a result, we could establish a high-throughput screening system using LC/MS/MS to detect oxidative metabolites and evaluated the action of the known inhibitor of IAA oxidation in rice.
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