2012 Fiscal Year Final Research Report

Method for Pin-Point Structure Analysis of Proteins by Fluorescence-Detected CD

Research Project

Project/Area Number	22550038
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Organic chemistry
Research Institution	Hiroshima University
Principal Investigator	NEHIRA Tatsuo 広島大学, 大学院・総合科学研究科, 助教 (60321692)
Co-Investigator(Renkei-kenkyūsha)	YAMAZAKI Takeshi 広島大学, 大学院・総合科学研究科, 教授 (30192397) ISHIDA Atsuhiko 広島大学, 大学院・総合科学研究科, 准教授 (90212886) HIRANO Tetsuo 広島大学, 大学院・総合科学研究科, 教授 (50228805)
Project Period (FY)	2010 – 2012
Keywords	構造有機化学
Research Abstract	Fluorescence-detected circular dichroism (FDCD) was applied to model proteins for the first time with the device that we have recently developed. Using metmyoglobin, a tryptophan-containing protein that gets denatured separately atsecondary and tertiary structure levels depending on the pH changes, it was unveiled that FDCD mainly observes the change of tertiary structure. Using a fluorescently-labeledcalmodulin, which alters its structure by the existence of calcium ions and/or binding peptides, it was demonstrated that FDCD can selectively observe the structure changes of the target protein.

(3 results)