Study on the Weak Interaction Regulating the Electronic Structure of Blue Copper Protein

2012 Fiscal Year Final Research Report

• Project/Area Number: 22550145
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Ibaraki University
• Principal Investigator: KOHZUMA Takamitsu 茨城大学, 理工学研究科, 教授 (50215183)
• Co-Investigator(Kenkyū-buntansha): UNNO Masaki 茨城大学, フロンティア応用原子科学研究センター, 准教授 (10359549)
• Project Period (FY): 2010 – 2012
• Keywords: ブルー銅蛋白質 / 弱い相互作用 / シュウドアズリン / X線結晶構造解析

Research Abstract

The Met16X pseudoazurin mutants were constructed, and spectroscopic and electrochemical properties of the mutant proteins were investigated. The electronic absorption and EPR spectra of Met16X variant proteins demonstrated the axial/rhombic components are rationally changeable by the characrer of the side chaing group of respective amino acids. Moreover the structure and function of Met16His can be modulated as a function of pH by the protonation/deprotonation of the newly introduced histidine moiety.

Research Products

• [Journal Article] X-ray Absorption Spectroscopy of an Unusual Plastocyanin from a Fern, Dryopteris crassirhizoma (2013)
  • Author(s): Hiromi Togashi, Junko Yano, Vittal K. Yachandra, and Takamitsu Kohzuma
  • Journal Title: Chem. Lett Volume: 42 Pages: 89-90
  • DOI: DOI:10.1246/cl.2013.89
  • Peer Reviewed
• [Journal Article] Direct electron transfer from pseudoazurin to nitrous oxide reductase in catalytic N_2O reduction (2012)
  • Author(s): K. Fujita, M. H.-Fujita, D. E. Brown, Y. Obara, F. Ijima, T. Kohzuma, D. M. Dooley
  • Journal Title: J. Inorg. Biochem Volume: 115 Pages: 163-173
  • URL: http:// dx.doi.org/10.1016/j.jinorgbio
  • Peer Reviewed
• [Journal Article] Theoretical studies on model reaction pathways of prostaglandinH2 isomerization to prostaglandin D2/E2 (2011)
  • Author(s): Naoto Yamaguchi, Tatsuya Naiki, Takamitsu Kohzuma, Toshikazu Takada, Fumihiko Sakata, Seiji Mori
  • Journal Title: Theor Chem Acc Volume: 128 Pages: 191-206
  • DOI: DOI:10.1007/s00214-010-0814-7.2012.07.013
  • Peer Reviewed
• [Journal Article] UV Resonance Raman Studies on the Activation Mechanism of Human Hematopoietic Prostaglandin D_2 Synthase by a Divalent Cation (2010)
  • Author(s): Yoshiko Uchida, Yoshihiro Urade, Seiji Mori, and Takamitsu Kohzuma
  • Journal Title: Mg^<2+> J. Bioinorg. Chem Volume: 104 Pages: 331-340
  • DOI: DOI:10.1016/j.jinorgbio.2009.12.003
  • Peer Reviewed
• [Journal Article] Czernuszewicz, Non-covalent interactions in blue copper protein probed by Met16 mutation and electronic and resonance Raman spectroscopy of Achromobacter cycloclastes pseudoazurin (2010)
  • Author(s): Marzena B. Fitzpatrick, Yuji Obara, Koyu Fujita, Doreen E. Brown, David M. Dooley, Takamitsu Kohzuma, Roman S
  • Journal Title: J. Bioinorg. Chem Volume: 104 Pages: 250-260
  • DOI: DOI:10.1016/j.jinorgbio.2009.11.004
  • Peer Reviewed
• [Presentation] Weak Interaction Effects on the Protein Stability of Blue Copper Protein, Pseudoazurin from Achromobacter cycloclastes IAM 1013 (2012)
  • Author(s): T. Yamaguchi, Y, Nihei, S. Asamura, D. Sutherland, M. Stillman, T. Kohzuma
  • Organizer: 11th European Biological Inorganic Chemistry Conference
  • Place of Presentation: Granada(スペイン)
  • Year and Date: 20120900
• [Presentation] Effect of "Proton Switch" Induced by the Newly Introduced Uncoordinated Histidine in the Vicinity of the Blue Copper Active Site: High Resolution X-ray Crystallographic, Spectroscopic, and Electrochemical Studies of Met16His Pseudoazurin (2012)
  • Author(s): Takamitsu Kohzuma, Sayaka Asamura, Yuko Nihei,and Maski Unno
  • Organizer: 金属の関連す生体関連反応シンポジウム
  • Place of Presentation: 金沢大学
  • Year and Date: 20120600
• [Presentation] ブルー銅タンパク質シュウドアズリンにおける弱い相互作用の効果 (2012)
  • Author(s): 山口峻英, 仁平裕子, 松儀可奈子、Duncan Sutherland、矢野淳子、Vittal Yachandra、Martin Stillman、高妻孝光
  • Organizer: 日本化学会第92春季年会
  • Place of Presentation: 慶應義塾大学日吉キャンパス
  • Year and Date: 20120300
• [Presentation] ブルー銅タンパク質シュウドアズリンにおける弱い相互作用の加成性 (2011)
  • Author(s): 山口峻英、仁平裕子、浅村紗矢香、Duncan Sutherland, Martin Stillman, 高妻孝光
  • Organizer: 第22回日本化学会茨城地区研究交流会
  • Place of Presentation: 東海村
  • Year and Date: 20111100
• [Presentation] Additivity of Weak Interaction Effect in a Blue Copper Protein, Met16His/Thr36Lys Pseudoauzrin (2011)
  • Author(s): T. Yamaguchi, Y. Nihei, S. Asamura, T. Kohzuma
  • Organizer: 3rd Georgian Bay International Conference on Bioinorganic Chemistry
  • Place of Presentation: Parry Sound(カナダ)
  • Year and Date: 20110500
• [Presentation] Alkaline Structural Transition of Laccase from Rhus vernicifera of Okukuji Area, Ibaraki Prefecture, Japan (2011)
  • Author(s): Tomonori Muroya, Rika Takahashi, Hideto Terakado, Masanori Kaminaga and Takamitsu Kohzuma
  • Organizer: 3rd Georgian Bay International Conference on Bioinorganic Chemistry
  • Place of Presentation: Parry Sound(カナダ)
  • Year and Date: 20110500
• [Presentation] Atomic Resolution X-ray crystallographic Analyses and Spectroscopic Studies of a Cytochrome (2011)
  • Author(s): Akiko Takashina, Masaki Unno and Takamitsu Kohzuma
  • Organizer: c' from Alcaligenes xylosoxidans
  • Place of Presentation: Parry Sound(カナダ)
  • Year and Date: 20110500
• [Presentation] X-ray Absorption Spectroscopic Studies of Blue Copper Proteins, Fern Plastocyanin and Pseudoazurin (2011)
  • Author(s): Hiromi Togashi, Sayaka Asamura, Junko Yano, Vittal Yachandra, Fuminori Yoshizaki, and Takamitsu Kohzuma
  • Organizer: 3rd Georgian Bay International Conference on Bioinorganic Chemistry
  • Place of Presentation: Parry Sound(カナダ)
  • Year and Date: 20110500
• [Presentation] Effect of the non-covalent weak interaction on the electronic structure of blue copper protein (2011)
  • Author(s): T. Kohzuma, Y. Nihei, S. Asamura, M. Unno, Y. Obara, T. Yamaguchi, K. Matsugi, R. S. Czernuszewicz , D. Sutherland, M. Stillman, and R. K. Szilagyi
  • Organizer: Parry Sound
  • Place of Presentation: Canda
  • Year and Date: 20110300
• [Presentation] Atomic resolution X-ray crystallographic analyses, spectroscopic and kinetic studies of a cytochrome c' from Alcaligenes xylosoxidans (2010)
  • Author(s): Akiko Takashina, Masaki Unnno, Takamitsu Kohzuma
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii Convetion Center(アメリカ)
  • Year and Date: 20101200
• [Presentation] Roman Czernuszewicz Structure and Functions of Non-Covalent Weak Interaction Probed with a Blue Copper Protein (2010)
  • Author(s): Takamitsu Kohzuma, Yuji Obara, Yuko Nihei, Koyu Fujita, Doreen E. Brown, David M. Dooley
  • Organizer: Met16X Pseudoazurin Variants, 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii Convetion Center(アメリカ)
  • Year and Date: 20101200
• [Presentation] Effect of Weak Interaction on the Electronic Structure and Electrochemical Properties of Pseudoazurin Met16X mutants (2010)
  • Author(s): Yuji Obara, Marzena B. Fitzpatrick, Roman S. Czernuszewicz, Takamitsu Kohzuma
  • Organizer: 2010 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii Convetion Center(アメリカ)
  • Year and Date: 20101200
• [Presentation] 超高分解能X線結晶構造解析によるヘムタンパク質シトクロームc'の特異的構造変化 (2010)
  • Author(s): 高階明子, 海野昌喜, 高妻孝光
  • Organizer: 第21回日本化学会関東支部茨城地区研究交流会
  • Place of Presentation: 東海
  • Year and Date: 20101100
• [Presentation] Regulation of the Structure and Protein Stability through the Weak Interaction in the 2nd Coordination Sphere of a Blue Copper Protein (2010)
  • Author(s): T. Kohzuma, Y. Nihei, S. Asamura, Y. Obara, and M. Unno
  • Organizer: Pseudoazurin, XI International Symposium
  • Place of Presentation: Kudowa(ポーランド)
  • Year and Date: 20100900
• [Presentation] Regulation of the structure and protein stability through the weak interaction in the 2nd coordination sphere of metalloproteins (2010)
  • Author(s): T. Kohzuma
  • Organizer: 10th European Biological Inorganic Chemistry Conference
  • Place of Presentation: Thessalonike(ギリシャ)
  • Year and Date: 20100900
• [Presentation] Atomic Resolution X-ray crystallographic Analyses and Spectroscopic Studies of a Cytochrome c' from Alcaligenes xylosoxidans (2010)
  • Author(s): 高階明子, 海野昌喜, 高妻孝光
  • Organizer: 第48回日本生物物理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 20100900
• [Presentation] Effect of Weak Interaction on the Electronic Structure and Electrochemical Properties of Pseudoazurin Met16His/His6Val double mutant (2010)
  • Author(s): 小原裕二、仁平裕子、大上利恵、高妻孝光
  • Organizer: 第48回生物物理学会年会
  • Place of Presentation: 東北大学
  • Year and Date: 20100900
• [Presentation] 弱い化学的相互作用によるタンパク質の電子状態制御 (2010)
  • Author(s): 高妻孝光、仁平裕子、浅村紗矢香、小原裕二、海野昌喜
  • Organizer: 日本化学会関東支部
  • Place of Presentation: 筑波大学
  • Year and Date: 20100700
• [Presentation] ブルー銅タンパク質シュウドアズリンにおける弱い相互作用の効果 (2010)
  • Author(s): 高妻孝光、仁平裕子、浅村紗矢香、小原裕二、海野昌喜
  • Organizer: 第37回生体分子科学討論会
  • Place of Presentation: 山口大学
  • Year and Date: 20100600
• [Book] 元素111の新知識 第2版増補版 (2013)
  • Author(s): 桜井 弘、荒野 泰、上山憲一、小谷 明、高妻孝光、佐治英郎、鈴木晋一郎、寺嶋孝仁、中山祐正、根矢三郎、羽場宏光、廣田 俊、藤井敏司、吉村哲彦 著
  • Total Pages: 463
  • Publisher: 講談社ブルーバックス