A novel biosensing mechanism using sensor kinase activity

2012 Fiscal Year Final Research Report

• Project/Area Number: 22550149
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Chemistry related to living body
• Research Institution: Nagoya University
• Principal Investigator: NAKAJIMA Hiroshi 名古屋大学, 大学院・理学研究科, 教授 (00283151)
• Project Period (FY): 2010 – 2012
• Keywords: 生体機能材料 / シグナル変換 / バイオセンシング

Research Abstract

The objective of this study is to produce a novel bio-sensing mechanism constituted by sensor-kinase and signal transduction system that we proposed in previous works. Sensor-kinase is a protein which senses an external stimulus such as a simple molecule or light to phosphorylate a particular protein and regulate its activity. In this study, we attempted to transform the output of sensor-kinase to an electronic signal. We have almost achieved the initial objective by using a transcriptional regulator in place of sensor-kinase as a sensory moiety.

Research Products

• [Journal Article] Thermodynamic effects of the alteration of the axial ligand on the unfolding of the thermostable cytochrome c (2013)
  • Author(s): Rabindra KB., Nakajima, H., Jitumani R., Watanabe, Y., Mazumdar, S.
  • Journal Title: Biochemistry Volume: 52 Pages: 1373-1384
  • DOI: DOI:10.1021/bi300982v
  • Peer Reviewed
• [Journal Article] Cytochrome c552 from Thermus Thermophilus Engineered for Facile Conversion of the Prosthetic Group (2011)
  • Author(s): Ibrahim, Sk. Md., Nakajima, H., Ramanathan, K., Takatani, N., Ohta, T., Naruta, Y., Watanabe, Y.
  • Journal Title: Biochemistry Volume: 50 Pages: 9826-9835
  • DOI: DOI:10.1021/bi201048e
  • Peer Reviewed
• [Journal Article] Protein Photoconductors and Photodiodes (2011)
  • Author(s): Tokita, Y., Yamada, S., Luo, W., Goto, Y., Ford, N., Nakajima, H., Watanabe, Y.
  • Journal Title: Angew. Chem. Int. Ed. Volume: 50 Pages: 11663-11666
  • DOI: DOI:10.1002/anie.201103341
  • Peer Reviewed
• [Presentation] Dynamic Control of Inter-proteins Interaction for Protein Based Signal Transduction System (2013)
  • Author(s): H. Nakajima
  • Organizer: Collaborative Conference on Materials Research 2013
  • Place of Presentation: Jeju, Korea
  • Year and Date: 20130600
• [Presentation] An artificial Signal Transduction System Consisting of Electron Transfer Proteins (2013)
  • Author(s): H. Nakajima
  • Organizer: Campus Asia 3rd Meeting
  • Place of Presentation: Nanjing, China
  • Year and Date: 20130300
• [Presentation] 金属タンパク質のダイナミクスを利用する機能の創出 (2013)
  • Author(s): 中島 洋
  • Organizer: 分子研研究会「生体配位化学の最前線と展望」
  • Place of Presentation: 岡崎
  • Year and Date: 20130200
• [Presentation] シグナルトランスデューサーの化学 -機能解明と応用- (2013)
  • Author(s): 中島 洋
  • Organizer: 分子研研究会「生物物質科学の展望」
  • Place of Presentation: 岡崎
  • Year and Date: 20130100
• [Presentation] A Signal Transduction Mechanism Inspired by Natural Sensor Protein (2012)
  • Author(s): 中島 洋
  • Organizer: JST 日印バイオ医学研究 ワークショップ
  • Place of Presentation: 東京
  • Year and Date: 20120200
• [Presentation] Engineering of Cytochrome c552 from a Thermophile (2011)
  • Author(s): H. Nakajima
  • Organizer: Nagoya University Global COE in Chemistry, 4th Annual Symposium
  • Place of Presentation: Nagoya
  • Year and Date: 20111100
• [Presentation] Signal Transduction System Consisting of Electron Transfer Proteins (2011)
  • Author(s): H. Nakajima
  • Organizer: ICBIC15
  • Place of Presentation: Vancouver, Canada
  • Year and Date: 20110700
• [Presentation] Protein Engineering of Cytochrome c552 from a thermophile . A scaffold for artificial peroxidase (2010)
  • Author(s): H. Nakajima
  • Organizer: 10th IRTG joint symposium
  • Place of Presentation: Munster, Germany
  • Year and Date: 20101100
• [Presentation] 電子伝達タンパク質-環境応答性分子複合体を用いた、シグナル変換の試み (2010)
  • Author(s): 中島 洋
  • Organizer: 第20回金属の関与する生体関連反応シンポジウム
  • Place of Presentation: 徳島
  • Year and Date: 20100600
• [Remarks] ホームページ
  • URL: http://bioinorg.chem.nagoya-u.ac.jp/