2012 Fiscal Year Final Research Report
Characterization of the protein complex, which is required for the chromatin establishment at the centromere
| Project/Area Number |
22570008
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Genetics/Genome dynamics
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| Research Institution | National Institute of Genetics |
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Principal Investigator |
HORI Tetsuya 国立遺伝学研究所, 分子遺伝研究系, 助教 (70550078)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 染色体 |
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| Research Abstract |
To examine the chromatin structure in centromere region, we tried to identify proteins that associate with the CENP-T/CENP-W complex based on high sensitive Mass Spectrometry combined with the immunoprecipitation. We identified a protein Eaf6 and we confirmed that this localized to the centromeres. We also identified a histone acethyltransferase as an Eaf6-interacting protein. To examine function of Eaf6, we established an Eaf6-conditional knockout cell line and found that localization of several centromere proteins were abolished in Eaf6-deficient cells based on SILAC analysis, indicating that the Eaf6 complex might be required for chromatin architecture of centromeres through the acethyltransferase activity of the Eaf6 complex.
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Research Products
(35 results)
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[Journal Article] "Chromosome engineering allows the efficient isolation of vertebrate neocentromeres."2013
Author(s)
Shang WH, Hori T, Martins NMC, Toyoda A, Misu S, Monma N, Hiratani I, Maeshima K, Ikeo K, Fujiyama A, Kimura H, Earnshaw WC, Fukagawa T
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Journal Title
Dev. Cell
Volume: 24
Pages: 635-348
DOI
Peer Reviewed
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[Journal Article] "The actin family member Arp6 and the histone variant H2A.Z are required for spatial positioning of chromatin in chicken cell nuclei."2012
Author(s)
Ohfuchi ME, Hori T, Tanabe H, Kitamura H, Matsuda R, Tone S, Hozak P, Habermann, FA, Hase J, Cremer C, Fukagawa T, Harata M
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Journal Title
J. Cell Sci
Volume: 125
Pages: 3739-3743
DOI
Peer Reviewed
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