2012 Fiscal Year Final Research Report

functional evolution of nebulin superfamily in vertebrates

Research Project

Project/Area Number	22570072
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Animal physiology/Animal behavior
Research Institution	Chiba University
Principal Investigator	TERASAKI Asako 千葉大学, 大学院・融合科学研究科, 講師 (30311616)
Co-Investigator(Kenkyū-buntansha)	KATSUYAMA Yu 神戸大学, 医学(系)研究科(研究院), 助教 (10359862)
Co-Investigator(Renkei-kenkyūsha)	KAMIGUCHI Hiroyuki 独立行政法人理化学研究所, チームリーダー (10233933) AKIYAMA Hiroki 独立行政法人理化学研究所, リサーチアソシエイト (40568854)
Project Period (FY)	2010 – 2012
Keywords	nebulin / 分子進化
Research Abstract	Nebulin superfamily proteins such as lasp, lasp-1, lasp-2, nebulin, nebulette, and N-RAP have similar domain structures (nebulin repeats, LIM domain and SH3 domain) and are thought to be generated from the same ancestral gene. This study tried to reveal their functional evolution in nerve and muscle tissues in vertebrates. We found tissue distribution of lasp-1 and lasp-2 were different in zebra fish. Localization of lasp-1 and lasp-2 were different in primary neurons of chicken and rat and siRNA of lasp-2 and dominant negative constructs of lasp-2 resulted morphological abnormality in filopodia in the primary neurons. Lasp-2 play roles in vesicle transport, cell-substrate attachment and peripheral astrocyte process in glial cells of chicken and rat.

(3 results)