2012 Fiscal Year Final Research Report
Elucidation of protein C-mannosylation mechanism
| Project/Area Number |
22570124
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Structural biochemistry
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
INAI Yoko 和歌山県立医科大学, 医学部, 助教 (20316087)
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| Co-Investigator(Kenkyū-buntansha) |
IHARA Yoshito 和歌山県立医科大学, 医学部, 教授 (70263241)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 翻訳後修飾 / C-マンノシル化 |
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| Research Abstract |
To better understand of mechanism of protein C-mannosylation, we tried to develop a simple approach to assess this modification of cell lines. We have used site-directed mutagenesis to alter the tryptophan, a predicted C-mannosylation site in TSR protein A, to alanine or phenylalanine. Both mutations resulted in decreased secretion of the protein, andthe mutants were retained in the endoplasmic reticulum. These results suggested the possibility that the secretion of the protein may representthe protein C-mannosylation in the cell lines.
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