2012 Fiscal Year Final Research Report
A regulation mechanism of homologous recombination by protein-protein interactions
| Project/Area Number |
22570147
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MIKAWA Tsutomu 独立行政法人理化学研究所, 遺伝制御科学特別研究ユニット, 専任研究員 (20321820)
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| Research Collaborator |
INOUE Jin 独立行政法人理化学研究所, 遺伝制御科学特別研究ユニット, 特別研究員 (10469893)
MASUDA Tokiha 独立行政法人理化学研究所, 遺伝制御科学特別研究ユニット, ジュニアリサーチアソシエイト
SHINOHARA Takeshi 独立行政法人理化学研究所, 遺伝制御科学特別研究ユニット, ジュニアリサーチアソシエイト
OKUI Saya 独立行政法人理化学研究所, 遺伝制御科学特別研究ユニット, 研修生
KASAGI Motochika 独立行政法人理化学研究所, 遺伝制御科学特別研究ユニット, 研修生
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| Project Period (FY) |
2010 – 2012
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| Keywords | 相同組換え / タンパク質間相互作用 / タンパク質-DNA間相互作用 / NMR |
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| Research Abstract |
Homologous recombinational repair (HRR) is an important pathway to maintain genomic stability. The RecF pathway is a bacterial HRR, in which RecA, RecF, RecO, RecR, and SSB proteins regulate HRR via their interactions. In this study, relationships between the interactions and regulation of HRR have been analyzed. As a result, a model mechanism of HRR regulation based on the protein-protein interaction was proposed.
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Research Products
(48 results)
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[Journal Article] High-resolution heteronuclear multidimensional NMR of proteins in living insect cells using a baculovirus protein expression system2013
Author(s)
Hamatsu, J., O'Donovan, D., Tanaka,T., Shirai, T., Hourai, Y., Mikawa, T.
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Journal Title
J Am Chem Soc
Volume: 135
Pages: 1688-91
DOI
Peer Reviewed
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[Journal Article] The double-stranded break-forming activity of plant SPO11s and a novel rice SPO11 revealed by a Drosophila bioassay2012
Author(s)
Shingu, Y., Tokai, T., Agawa, Y., Toyota, K., Ahamed, S., Kawagishi-Kobayashi, M., Komatsu, A., Mikawa, T., (他4名)
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Journal Title
BMC Mol Biol
Volume: 13
Pages: 1
DOI
Peer Reviewed
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[Journal Article] Vital roles of the second DNA-binding site of Rad52 protein in yeast homologous recombination2011
Author(s)
Arai, N., Kagawa, W., Saito, K., Shingu, Y., Mikawa, T., Kurumizaka, H. & Shibata, T.
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Journal Title
J Biol Chem
Volume: 286
Pages: 17607-17
DOI
Peer Reviewed
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[Journal Article] A mechanism for single-stranded DNA-binding protein (SSB) displacement from single-stranded DNA upon SSB-RecO interaction2011
Author(s)
Inoue, J., Nagae, T., Mishima, M., Ito, Y., Shibata, T. & Mikawa, T.
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Journal Title
J Biol Chem
Volume: 286
Pages: 6720-32
DOI
Peer Reviewed
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[Journal Article] NMR protein structure determination in living E. coli cells using nonlinear sampling2010
Author(s)
Ikeya, T., Sasaki, A., Sakakibara, D., Shigemitsu, Y., Hamatsu, J., Hanashima, T., Mishima, M., Yoshimasu, M., Hayashi, N., Mikawa, T., (他6名)
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Journal Title
Nat Protoc
Volume: 5
Pages: 1051-60
DOI
Peer Reviewed
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