2012 Fiscal Year Final Research Report
Studies on a role of autophagy on the catabolism of sialylated oligosaccharides
| Project/Area Number |
22570148
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Functional biochemistry
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
SUZUKI Tadashi 独立行政法人理化学研究所, 糖鎖代謝学研究チーム, チームリーダー (90345265)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 遊離糖鎖 / オートファジー / シアル酸 / 糖鎖代謝 / 細胞質 / リソソーム |
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| Research Abstract |
Previously, we showed that in Atg5^<-/-> cells, sialyl oligosaccharides specifically accumulated in the cytosol. Accumulation of these glycans was observed under non-starved conditions, suggesting that non-induced, basal autophagy is essential for their catabolism. Interestingly, once accumulated in the cytosol, sialyl glycans cannot be efficiently catabolized by resumption of the autophagic process, suggesting that functional autophagy is important for preventing sialyl oligosaccharides from accumulating in the cytosol. Moreover, knockdown of sialin, a lysosomal transporter of sialic acids, resulted in a significant reduction of sialyl oligosaccharides, implying that autophagy affects the substrate specificity of this transporter. This study thus provides a surprising link between basal autophagy and catabolism of N-linked glycans.
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