Molecular mechanism of a light-regulated transcription factor,aureochrome.

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570162
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Biophysics
• Research Institution: Osaka University
• Principal Investigator: HISATOMI Osamu 大阪大学, 大学院・理学研究科, 准教授 (60231544)
• Co-Investigator(Renkei-kenkyūsha): KATAOKA Hironao 東北大学, 情報公開センター, 研究員 (30108568)
• Project Period (FY): 2010 – 2012
• Keywords: 光制御転写因子 / LOVドメイン / DNA結合

Research Abstract

To understand the molecular mechanism of a blue light (BL)-regulated transcription factor,aureochrome (AUREO), we prepared six kinds of recombinant AUREOs. BL appeared to induce a shift of the α-helical structure of the LOV domain to a β-sheet structure, and an approximately 5% increase in the hydrodynamic radius of recombinant AUREOs. Our results support a schema where BL-induced changes in the LOV domain may cause global conformational changes in the bZIP and/or the linker of AUREO.

Research Products

• [Journal Article] Blue light-induced conformational changes in a light-regulated transcription factor, aureochrome-1. (2013)
  • Author(s): Hisatomi, O., Takeuchi, K., Zikihara, K., Ookubo, Y., Nakatani, Y., Takahashi, F., Tokutomi, S., Kataoka, H.
  • Journal Title: Plant Cell Physiol. Volume: 54(1) Pages: 93-106
• [Journal Article] Hematological- and neurological-expressed sequence 1 gene products in progenitor cells during newt retinal development (2012)
  • Author(s): Goto, Y., Tokunaga, F., Hisatomi, O
  • Journal Title: Stem Cells International Volume: vol. 2012, Article ID 436042 Pages: 6
• [Journal Article] Photoreactions of aureochrome-1 (2011)
  • Author(s): Toyooka, T., Hisatomi, O., Takahashi, F., Kataoka, H., Terazima, M.
  • Journal Title: Biophys J. Volume: 100(11) Pages: 2801-2809
• [Journal Article] Hydrogen bonding natures of water molecules in aqueous solutions of glycerol by Attenuated Total Reflection Infrared spectroscopy (2011)
  • Author(s): Kataoka, Y., Kitadai, N., Hisatomi, O., Nakashima, S
  • Journal Title: Applied spectroscopy Volume: 65(4) Pages: 436-441
• [Presentation] Blue-light induced conformational change of recombinant AUREO1 (2012)
  • Author(s): Hisatomi, O., Takeuchi, K., Takahashi, F. and Kataoka, H.
  • Organizer: 15th International Conference on the Cell and Molecular Biology of Chlamydomonas.
  • Place of Presentation: Potsdam, Germany.
  • Year and Date: 20120605-10
• [Presentation] Expression and characterization of recombinant aureochrome-1 (2012)
  • Author(s): Hisatomi, O., Takeuchi, K., Takahashi, F. and Kataoka, H.
  • Organizer: The 1st International Symposium on Plant Environmental Sensing
  • Place of Presentation: Todaiji Temple Cultural Center, Nara, Japan
  • Year and Date: 20120319-21
• [Presentation] Blue-light induced conformational change of a transcription factor, AUREO1. (2011)
  • Author(s): Hisatomi, O., Takeuchi, K., Nakatani, Y., Takahashi, F., Kataoka, H
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋大学(東山キャンパス)
  • Year and Date: 20110922-24
• [Presentation] FTIR study of Aureochrome that possesses LOV domain at the C-terminus. (2011)
  • Author(s): Ito, S., Zhang, Yu., Iwata, T., Hisatomi, O., Takahashi, F., Kataoka, H., Kandori, H
  • Organizer: 日本生物物理学会第50回年会
  • Place of Presentation: 名古屋大学(東山キャンパス)
  • Year and Date: 20110922-24
• [Presentation] DNA binding properties of a bHLH transcription factor fused with green fluorescent protein (2011)
  • Author(s): Hisatomi, O. and Furuya, K
  • Organizer: 8th International Congress of Comparative Physiology and Biochemistry (ICCPB2011)
  • Place of Presentation: Nagoya Congress Center, Aichi, Japan
  • Year and Date: 20110531-0605
• [Presentation] イモリの網膜再生に関与するbHLH型転写因子の解析 (2011)
  • Author(s): 古家景悟、久冨修
  • Organizer: 日本動物学会第82回大会
  • Place of Presentation: 旭川大雪クリスタルホール(北海道)
  • Year and Date: 2011-09-21
• [Presentation] Photoreaction of a light-regulated transcription factor, AUREO1 (2011)
  • Author(s): Hisatomi, O., Takeuchi, K., Murakami, T.,Takahashi, F., Kataoka, H.
  • Organizer: 日本生物物理学会第49回年会
  • Place of Presentation: 兵庫県立大学(兵庫県)
  • Year and Date: 2011-09-18
• [Presentation] Characterization of a light-regulated transcription factor, AUREO1, expressed in E. coli (2011)
  • Author(s): Hisatomi, O., Murakami, T., Furuya, K., Takahashi, F. and Kataoka, H
  • Organizer: 5th Asia and Oceania Conference on Photobiology (AOCP2011)
  • Place of Presentation: Nara Prefectural New Public Hall, Nara, Japan
  • Year and Date: 2011-07-31
• [Presentation] Hydration behavior of biomolecules as studied by attenuated total reflection infrared spectroscopy. (2010)
  • Author(s): Kataoka, Y., Kitadai, N., Hisatomi, O., Nakashima, S
  • Organizer: 平成22年度日本分光学会年次講演会
  • Place of Presentation: 京大(吉田キャンパス)
  • Year and Date: 20101118-20
• [Presentation] 網膜形成に関与するbHLH型転写因子の解析 (2010)
  • Author(s): 久冨修、古家景悟、楳村直義
  • Organizer: 日本動物学会第81回大会
  • Place of Presentation: 東大(駒場キャンパス)
  • Year and Date: 20100923-25
• [Presentation] Analyses of a light-regulated transcription factor, AUREO1, expressed in E.coli. (2010)
  • Author(s): Hisatomi, O., Ohata, R., Furuya, K., Takahashi, F., Kataoka, H
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大(川内キャンパス)
  • Year and Date: 20100920-22
• [Presentation] Analyses of DNA binding properties of a bHLH transcription factor using site-directed mutants. (2010)
  • Author(s): Furuya, K., Hasegawa, K., Hisatomi, O
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大(川内キャンパス)
  • Year and Date: 20100920-22
• [Presentation] Attenuated Total Reflection Infrared spectroscopy of water molecules in the presence of biomolecules (2010)
  • Author(s): Kataoka, Y., Kitadai, N., Nakashima, S. , Hisatomi, O
  • Organizer: 日本生物物理学会第48回年会
  • Place of Presentation: 東北大(川内キャンパス)
  • Year and Date: 20100920-22
• [Presentation] 大量発現系を用いた光制御転写因子オーレオクロームの解析 (2010)
  • Author(s): 久冨修、古家景悟、高橋文雄、片岡博尚
  • Organizer: 光生物学協会講演会
  • Place of Presentation: 阪大(吹田キャンパス)
  • Year and Date: 20100810-11
• [Remarks]
  • URL: http://gabriel.ess.sci.osaka-u.ac.jp/html/hisatomi/hisatomi-jp.html