2012 Fiscal Year Final Research Report
Analysis of the mechanism of histone methylation by using genetic approaches
| Project/Area Number |
22570176
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
HAYASHI Aki 独立行政法人理化学研究所, クロマチン動態研究チーム, 研究員 (80399534)
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| Co-Investigator(Renkei-kenkyūsha) |
NAKAYAMA Jun-ichi 名古屋市立大学, システム自然科学研究科, 准教授 (60373338)
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| Project Period (FY) |
2010 – 2012
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| Keywords | ヘテロクロマチン / RNAi機構 / ヒストン修飾 / HP1 |
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| Research Abstract |
Epigenetics plays an important role for regulation of gene expression during developmental stages in eukaryote. To understand the molecular mechanism of epigenetic regulation, I analyzed the mechanism of histone modification at heterochromatic region of fission yeast by using genetic approaches. I obtained two uncharacterized genes involved in the formation of heterochromatin by screening. One of the genes, Ers1, binds both of heterochromatin protein 1 (HP1) and an RNA helicase Hrr1 functioned in RNAi pathway. Genetic and biochemical analysis revealed that Ers1 recruits the RNAi machinery to heterochromatic region and facilitates siRNA generation through the binding of HP1.
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