2012 Fiscal Year Final Research Report
Establishment of embryonic stem cell culture for high efficient generation of transgenic chick
| Project/Area Number |
22570177
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Molecular biology
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| Research Institution | The Institute of Physical and Chemical Research |
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Principal Investigator |
MAK Siu Shan 独立行政法人理化学研究所, 感覚器官発生研究室, 研究員 (40442967)
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| Co-Investigator(Kenkyū-buntansha) |
RAJ Ladher 独立行政法人理化学研究所, 感覚器官発生研究室, 特別主管研究員 (70392173)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 組換え / 胚性幹細胞 |
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| Research Abstract |
In this project, we set out to establish pluripotent cell culture in the zebra finch species. We found that finch eggs were laid at an earlier stage than chick. There were significant differences between the unincubated eggs of these two species in terms of cell identity and cell behaviour in vitro. As a consequence dissociated finch cells, but not chick cells, produced cells that expressed various stem cell markers under a defined culture condition. We reported for the first time, a detailed molecular analysis of post-morula avian embryos and a methodology for culturing such early embryos and a feeder-free serum-free culture condition that could obtain stem cell-like cells.
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