2013 Fiscal Year Final Research Report
Analyses of a novel function for SNAP-23 in phagosome maturation
| Project/Area Number |
22570189
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Tottori University (2012-2013)
Fukushima Medical University (2010-2011) |
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Principal Investigator |
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| Co-Investigator(Renkei-kenkyūsha) |
WADA Ikuo 福島県立医科大学, 医学部, 教授 (40182969)
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| Project Period (FY) |
2010-04-01 – 2013-03-31
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| Keywords | SNAREタンパク質 / ファゴサイトーシス / ファゴソーム / 膜融合 / マクロファージ / 生体防御 |
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| Research Abstract |
Phagosome maturation in phagocytes is thought to be accomplished by membrane fusion of phagosome with other organelles, but the molecular mechanism in this process remains unknown.
In this study, we have found SNAP-23, a plasma membrane-localized SNARE, functions not only in phagocytosis but also in phagosome maturation processes such as production of reactive oxygen species and acidification within phagosomes and fusion of phagosome with lysosome. To trace a conformational change of SNAP-23 during fusion event, we established a single-molecule fluorescence resonance energy transfer system for SNAP-23. Using this system, we have found a forming SNARE complex with VAMP7 on phagosomal membrane and a novel complex including VAMP5 and syntaxin3 on plasma membrane. This suggests that SNAP-23 mediates the membrane fusion of phagosome with lysosome by forming a SNARE complex with VAMP7. Unfortunately, we have never found a positive function of VAMP5 in phagosome formation and maturation.
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[Journal Article] Quantitative proteomi cs reveals that only a subset of the endoplasmic reticulum contributes to the phagosome2012
Author(s)
Campbell-Valois FX, Trost M, Chema li M, Dill BD, Laplante A, Duclos S, Sadeghi S, Rondeau C, Morrow IC, Bell C, Hatsuzawa K, Thibault P, Desjardins M
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Journal Title
Mol Cell Proteomics
Volume: 11
Pages: M111.016378
DOI
Peer Reviewed
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