2012 Fiscal Year Final Research Report
Molecular mechanism of microtubule stabilization by plus-end tracking proteins
| Project/Area Number |
22570190
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Cell biology
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| Research Institution | Yokohama City University |
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Principal Investigator |
HAYASHI Ikuko 横浜市立大学, 大学院・生命医科学研究科, 准教授 (80464527)
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| Co-Investigator(Renkei-kenkyūsha) |
YASUNAGA Takuo 九州工業大学, 大学院・情報工学研究院, 教授 (60251394)
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| Project Period (FY) |
2010 – 2012
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| Keywords | x線結晶構造解析 / 電子顕微鏡解析 / 細胞骨格 / 細胞遊走 |
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| Research Abstract |
Microtubule plus-endtracking protein CLASP proteins playa critical role in chromosomal segregation and cell migration. We determined the crystal structures of two microtubule-binding TOG domains in CLASP2. By mutational analysis, we showed that bothTOG domains are essential for CLASP2 binding to microtubules and are able to stabilizemicrotubules to a maximum extent when CLASP2 forms a complex with another microtubule-binding protein EB1.
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