The molecular analysis of the development of cerebellum by using zebrafish mutants

2012 Fiscal Year Final Research Report

• Project/Area Number: 22570201
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Developmental biology
• Research Institution: Nagoya University
• Principal Investigator: SHIMIZU Takashi 名古屋大学, 生物機能開発利用研究センター, 准教授 (50324760)
• Project Period (FY): 2010 – 2012
• Keywords: 細胞分化 / 神経分化 / 小脳 / 顆粒細胞

Research Abstract

To understand the genes that control the development of cerebellar neurons, we investigated the zebrafish mutants gazami(gaz) and shiomaneki(sio).The gaz mutant larvae showed a strong reduction of granule cells. By the positional cloning, we mapped the gaz mutation to the gene encoding a nuclear protein, cranio facial development protein1(cfdp1). In the anterior neuroectoderm, the number of apoptotic cells was increased in the mutant larvae. We also found that proliferating cells were increased in mutants at 4 dpf. Our findings suggest that Cfdp1 function in the differentiation from the neural progenitors and/or proliferation of neural progenitor cells.The sio mutants displayed mistargeted axon of granule cells. The sio mutation mapped to the gene encodes type IV collagen a6 whish serve as a component of basement membrane.The sio larvae showed the disruption of basement membrane structure. These results suggest that the interaction between neuron and basement membrane is important for the axogenesis of cerebellar granule cells.

Research Products

• [Journal Article] Development of the cerebellum and cerebellar neural circuits. (2012)
  • Author(s): Hibi M. and Shimizu T.
  • Journal Title: Dev. Neurobiol. Volume: 72(3) Pages: 282-301
  • Peer Reviewed
• [Journal Article] Atypical PKC regulates primary dendrite specification of cerebellar Purkinje cells by localizing Golgi apparatus (2010)
  • Author(s): Tanabe K., Kani S., Shimizu T., Bae Y.-K., Abe T., and Hibi M.
  • Journal Title: J Neurosci. Volume: 30 Pages: 16983-16992
  • Peer Reviewed
• [Journal Article] Proneural gene-linked neurogenesis in zebrafish cerebellum. (2010)
  • Author(s): Kani S., Bae Y.-K., Shimizu T., Tanabe K., Satou C., Parsons P.J., Scott E., Higashijima S. and Hibi M.
  • Journal Title: Dev. Biol. Volume: 343 Pages: 1-17
  • Peer Reviewed
• [Journal Article] Zinc finger genes Fezf1 and Fezf2 control neuronal differentiation by repressing Hes5 expression in forebrain (2010)
  • Author(s): Shimizu, T., Nakazawa, M., Kani, S., Bae, Y.-K., Shimizu, T., Kageyama, R., and Hibi, M.
  • Journal Title: Development Volume: 137 Pages: 1875-1885
  • Peer Reviewed
• [Journal Article] a motor protein linker, controls dorsal determination. (2010)
  • Author(s): Nojima, H., Rothhamel, S., Shimizu, T., Kim, C.H., Yonemura, S., Marlow,F.L., and Hibi, M. Syntabulin
  • Journal Title: Development Volume: 137 Pages: 923-933
  • Peer Reviewed
• [Presentation] A nuclear protein Cfdp1 is involved in differentiation of granule cells in cerebellum (2013)
  • Author(s): 清水貴史
  • Organizer: 第6回神経発生討論会
  • Place of Presentation: 和光
  • Year and Date: 20130300
• [Presentation] Role of basement membrane in axogenesis of cerebellar granule cells in zebrafish (2012)
  • Author(s): Hibi, M., Takeuchi, M., Yonemura, S., Asakawa, K., Kawakami, K., and Shimizu, T.
  • Organizer: Asia-Pacific Developmental Biology Conference 2012
  • Place of Presentation: 台北、台湾
  • Year and Date: 20121000
• [Presentation] Role of basement membrane in axogenesis of cerebellar granule cells (2012)
  • Author(s): Takeuchi, M., Yamaguchi, S., Yonemura, S, Asakawa, K., Kawakami, K., Takeda, S., Shimizu, T., and Hibi, M.
  • Organizer: 第18回小型魚類研究会
  • Place of Presentation: 京都
  • Year and Date: 20120900
• [Presentation] Role of type IV collagen in axogenesis of cerebellar granule cells in zebrafish (2012)
  • Author(s): Takeuchi, M., Shimizu, T., Asakawa, K., Kawakami, K., Yonemura, S., and Hibi, M.
  • Organizer: 10th International Conference on Zebrafish Development and Genetics.
  • Place of Presentation: Madison, USA
  • Year and Date: 20120600
• [Presentation] IV型コラーゲンはゼブラフィッシュにおける小脳顆粒細胞の軸索伸長に必要である (2012)
  • Author(s): 竹内未紀、清水貴史、浅川和秀、川上浩一、米村重信、日比正彦
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
  • Year and Date: 20120600
• [Presentation] Role of type IV collagen in axogenesis of cerebellar granule cells in zebrafish (2012)
  • Author(s): Takeuchi, M., Shimizu, T., Asakawa, K., Kawakami, K., Yonemura, S., and Hibi, M.
  • Organizer: 第45回日本発生生物学会合同大会
  • Place of Presentation: 神戸
  • Year and Date: 20120500
• [Presentation] Genetic control for development of cerebellar neurons and neural circuits in zebrafish (2011)
  • Author(s): Takeuchi, M., Shimizu, T., Kani, S., Bae, Y.-K., Tanabe, K., Kusuda, R.*, Asakawa, K., Kawakami, K., and Hibi, M.
  • Organizer: 第17回小型魚類研究会
  • Place of Presentation: 三島
  • Year and Date: 20110900
• [Presentation] Development of cerebellar neural circuits in zebrafish and medaka (2011)
  • Author(s): Hibi, M. and Shimizu, T.
  • Organizer: 第34回日本神経科学大会
  • Place of Presentation: 横浜
  • Year and Date: 20110900
• [Presentation] Genetic control of development of cerebellar neurons and neural circuits in zebrafish (2011)
  • Author(s): Shimizu, T., Kani, S., Bae, Y.-K.,Tanabe, K., Kusuda, R., and Hibi, M.
  • Organizer: 第44回日本発生生物学会年会
  • Place of Presentation: 沖縄
  • Year and Date: 20110500
• [Presentation] Neurogenesis in Zebrafish Cerebellum (2010)
  • Author(s): 清水 貴史、可児 修一、Bae Young-Ki、田辺 光志、日比 正彦
  • Organizer: 第15回小型魚類研究会
  • Place of Presentation: 埼玉
  • Year and Date: 20100900
• [Presentation] Neurogenesis in Zebrafish Cerebellum (2010)
  • Author(s): Shimizu, T., Kani, S., Bae, Y.-K., Tanabe, K., and Hibi, M.
  • Organizer: 8th International Conference on Zebrfish Development and Genetics
  • Place of Presentation: Madison, USA
  • Year and Date: 20100600
• [Remarks]
  • URL: http://bbc.agr.nagoya-u.ac.jp/~junkei/i ndex.html