2012 Fiscal Year Final Research Report
Analyses of the role of Shh-expressing cells during mammalian cortical development
| Project/Area Number |
22570209
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Developmental biology
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| Research Institution | Doshisha University |
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Principal Investigator |
MOTOYAMA Jun 同志社大学, 脳科学研究科, 教授 (70321825)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 遺伝学 / 神経科学 / 脳神経 / 発生分化 / シグナル伝達 |
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| Research Abstract |
The objective is to identify the role of Sonic hedgehog (Shh) during mammalian cortical development. We identified the source of Shh protein during cortical development using EGFP expression under Shh-creERT2/beta-geo-EGFP (ZEG) system under the administration of tamoxifen at both E13.5 and E14.5. Shh expressing cells were limited population of post-mitotic cortical neuronal cells in the intermedate zone. To identify the role of these cells, we induced apoptosis in the Shh expressing cells by using mice with Shh-creERT2/loxP-STOP-loxP-DTA background. By the ablation of the Shh-expressing cells, we detected significant reduction of the differentiation from neural stem cells, indicating that the Shh-expressing cells in the intermediate zone is required for the differentiation of neural stem cells into the mitotic progenitors.
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