2012 Fiscal Year Final Research Report

Analyses of the role of Shh-expressing cells during mammalian cortical development

Research Project

Project/Area Number	22570209
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Developmental biology
Research Institution	Doshisha University
Principal Investigator	MOTOYAMA Jun 同志社大学, 脳科学研究科, 教授 (70321825)
Project Period (FY)	2010 – 2012
Keywords	遺伝学 / 神経科学 / 脳神経 / 発生分化 / シグナル伝達
Research Abstract	The objective is to identify the role of Sonic hedgehog (Shh) during mammalian cortical development. We identified the source of Shh protein during cortical development using EGFP expression under Shh-creERT2/beta-geo-EGFP (ZEG) system under the administration of tamoxifen at both E13.5 and E14.5. Shh expressing cells were limited population of post-mitotic cortical neuronal cells in the intermedate zone. To identify the role of these cells, we induced apoptosis in the Shh expressing cells by using mice with Shh-creERT2/loxP-STOP-loxP-DTA background. By the ablation of the Shh-expressing cells, we detected significant reduction of the differentiation from neural stem cells, indicating that the Shh-expressing cells in the intermediate zone is required for the differentiation of neural stem cells into the mitotic progenitors.

(1 results)