2013 Fiscal Year Final Research Report
Diversity and evolution of mitochondrial DNA-binding protein, Abf2
| Project/Area Number |
22570213
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Evolutionary biology
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| Research Institution | Yamaguchi University |
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Principal Investigator |
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| Project Period (FY) |
2010-04-01 – 2014-03-31
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| Keywords | 酵母 / ミトコンドリア / ミトコンドリア核様体 / DNA結合タンパク質 / Abf2 |
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| Research Abstract |
In order to reveal the evolution of mitochondrial DNA-binding protein, Abf2 in the budding yeast, Saccharomyces cerevisiae, we identified Mhb1 and Cmb1 proteins as novel mitochondrial DNA-binding proteins from the mitochondrial nucleoids of the budding yeast Y. lipolytica and the fission yeast S. pombe, respectively. Both of Mhb1 and Cmb1 proteins retain one HMG domain at the C-terminal region of the protein. We revealed the DNA-binding characters of both proteins. We raised a polyclonal antibody against Cmb1 and demonstrated that Cmb1 is localized to mitochondria by immunofluorescence microscopy. We also demonstrated that Cmb1 complements the function of Abf2 in abf2-deficient cells of S. cerevisiae.
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[Journal Article] Aldehyde dehydrogenase, Ald4p, is a major component of mitochondrial fluorescent inclusion bodies in the yeast Saccharomyces cerevisiae2014
Author(s)
Misonou, Y., Kikuchi, M., Sato, H., Inai, T., Kuroiwa, T., Tanaka, K., Miyakawa
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Journal Title
Biology Open
Volume: 3
Pages: 387-396
Peer Reviewed
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[Journal Article] Heterocyclic bismuth carboxylates based on a diphenyl sulfone scaffold : Synthesis and antifungal activity against Saccharomyces cerevisiae2013
Author(s)
Murafuji, T., Kitagawa, K., Yoshimatsu, D., Kondo, K., Ishiguro, K., Tsunashima, R., Miyakawa, I., and Mikata, Y.
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Journal Title
European Journal of Medicinal Chemistry
Volume: 63
Pages: 531-535
Peer Reviewed
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