Pathway engineering of ethanologenic bacteria based on HiCEP (High Coverage Expression Profiling)analysis

2012 Fiscal Year Final Research Report

• Project/Area Number: 22580084
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Applied microbiology
• Research Institution: Tottori University
• Principal Investigator: YANASE Hideshi 鳥取大学, 大学院・工学研究科, 教授 (20158033)
• Project Period (FY): 2010 – 2012
• Keywords: バイオマスエネルギー / 微生物遺伝学 / 代謝工学

Research Abstract

To improve the tolerance of Zymobacter palmae to various stress during cellulosic bioethanol fermentation, we have been engineering genetically a robust Zb. palmae based on the gene expression profiling responding to fermentation stress by HiCEP and DNA microarray analyses. We found the first mechanism acquiring the acid tolerance depended on the amino acid biosynthetic pathway.

Research Products

• [Journal Article] Expression and surface display of Cellulomonas endoglucanase in the ethanologenic bacterium Zymobacter palmae (2012)
  • Author(s): Kojima, M., Akahoshi, T., Okamoto, K.,and Yanase, H.
  • Journal Title: Appl. Microbiol. Biotechno Volume: 96
  • Peer Reviewed
• [Journal Article] Ethanol production from wood hydrolysate using genetically engineered Zymomonas mobilis (2012)
  • Author(s): Yanase, H., Miyawaki, H., Sakurai, M.,Kawakami, A., Matsumoto, M., Haga, K.,Kojima, M., and Okamoto, K.
  • Journal Title: Appl. Microbiol. Biotechnol. Volume: 94
  • Peer Reviewed
• [Journal Article] Characterization of the protein gp1 from bacteriophage In 93 (2011)
  • Author(s): Matsushita I., and Yanase H.
  • Journal Title: Biological Lett Volume: 48(1) Pages: 47-56
  • Peer Reviewed
• [Journal Article] Direct ethanol production from cellulosic materials by Zymobacter palmae carrying Cellulomonas and Ruminococcus -glucosidase
  • Author(s): Kojima, M., Okamoto, K., and Yanase,H.
  • Journal Title: Appl. Microbiol. Biotechno
  • DOI: DOI:10.1007/s00253-013-4874-1
  • Peer Reviewed
• [Presentation] C5・C6糖並行発酵性Zymobacter palmaeのキシロース発酵性強化 (2012)
  • Author(s): 滝上、近藤、奥田、岡本、原田、簗瀬
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-10-26
• [Presentation] ラッカーゼ処理によるリグニン過分解物発酵阻害の低減効果 (2012)
  • Author(s): 伊原、高橋、杉岡、岡本、原田、簗瀬
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-10-26
• [Presentation] Cellvibrio japonicus由来セルラーゼ遺伝子のクローニングと発酵細菌内での分泌発現 (2012)
  • Author(s): 小島、幡部、玉井、岡本、原田、簗瀬
  • Organizer: 第64回日本生物工学会大会
  • Place of Presentation: 神戸国際会議場
  • Year and Date: 2012-10-26
• [Presentation] バイオマスエネルギー技術の現状と将来 (2012)
  • Author(s): 簗瀬英司
  • Organizer: 日本機械学会中国四国支部第114講習会
  • Place of Presentation: 鳥取
  • Year and Date: 2012-01-20
• [Presentation] Ethanol production from pretreated eucalyptus wood chips by simultaneous saccharification and fermentation RAFT (2011)
  • Author(s): Matsushita H, Yanase H, Daidai M, Sego H,Yasutani N, Habu K, Kikuta H, Yasue Y,Watanabe T
  • Organizer: Recent Advances in Fermentation Technology
  • Place of Presentation: 米国
  • Year and Date: 2011-11-08
• [Presentation] Metabolic engineering of ethanologenic bacteria capable of producing cellulosic bioethanol (2011)
  • Author(s): Yanase,H.
  • Organizer: International Union of Microbiological Soceities 2011 Congress
  • Place of Presentation: 札幌
  • Year and Date: 2011-09-09
• [Patent(Industrial Property Rights)] マンノース発酵性ザイモバクター属形質転換微生物 (2011)
  • Inventor(s): 簗瀬英司、岡本賢治
  • Industrial Property Rights Holder: 国立大学法人鳥取大学
  • Industrial Property Number: 特許第4686709号
  • Acquisition Date: 2011-02-25
• [Patent(Industrial Property Rights)] 染色体組み込みマンノース発酵性ザイモモナス属細菌 (2010)
  • Inventor(s): 簗瀬英司、岡本賢治
  • Industrial Property Rights Holder: 国立大学法人鳥取大学
  • Industrial Property Number: 特許第4528972号
  • Acquisition Date: 2010-06-18