2012 Fiscal Year Final Research Report
Characterization of indolocarbazole biosynthetic enzymes; for the application to
| Project/Area Number |
22580089
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied microbiology
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| Research Institution | The University of Tokyo (2012)
Toyama Prefectural University (2010-2011) |
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Principal Investigator |
ONAKA Hiroyasu 東京大学, 大学院・農学生命科学研究科, 特任教授 (80315829)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 遺伝学 / 応用微生物 / 抗生物質 / 発酵 / バイオテクノロジー |
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| Research Abstract |
Indolocarbazole (ICZ) skeleton is biosynthesized from tryptophane as a precursor byfour biosynthetic enzymes. In this study, the biosynthetic enzymes involved instaurosporine, one of ICZ are characterized. StaD is a coupling enzyme of twomolecules of indolepyruvic acid. We detected the intermediate of the enzymaticreaction and determine the chemical structure. We then analyzed how StaC catalyzesthe formation of K252c by using of mutated StaC.
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