2012 Fiscal Year Final Research Report

Structure-function relationship of endo-type enzymes involved in plant cell surface proteoglycans

Research Project

Project/Area Number	22580110
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Applied biochemistry
Research Institution	National Agriculture and Food Research Organization
Principal Investigator	KANEKO Satoshi 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品バイオテクノロジー研究領域, 主任研究員 (90343821)
Project Period (FY)	2010 – 2012
Keywords	酵素利用学 / 糖質関連酵素
Research Abstract	An endo-.-1,6-galactanase which act on thickening agent arabinogalactan was obtained from Streptomyces avermitilis. The substrate specificity and the crystal structure of the recombinant enzyme were elucidated. To understand the detail mechanism of substrate specificity of the endo-.-1,6-galactanase, the other endo-.-1,6-galactanase which consist from distinctive amino acid sequence was cloned and crystal of the recombinant enzyme was obtained. However, quality of the crystal was not enough to solve structure so that the mechanism of substrate specificity of the endo-.-1,6-galactanase could not be elucidated.

(2 results)