2012 Fiscal Year Final Research Report
Structure-function relationship of endo-type enzymes involved in plant cell surface proteoglycans
| Project/Area Number |
22580110
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Applied biochemistry
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| Research Institution | National Agriculture and Food Research Organization |
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Principal Investigator |
KANEKO Satoshi 独立行政法人農業・食品産業技術総合研究機構, 食品総合研究所・食品バイオテクノ ロジー研究領域, 主任研究員 (90343821)
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| Project Period (FY) |
2010 – 2012
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| Keywords | 酵素利用学 / 糖質関連酵素 |
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| Research Abstract |
An endo-.-1,6-galactanase which act on thickening agent arabinogalactan was obtained from Streptomyces avermitilis. The substrate specificity and the crystal structure of the recombinant enzyme were elucidated. To understand the detail mechanism of substrate specificity of the endo-.-1,6-galactanase, the other endo-.-1,6-galactanase which consist from distinctive amino acid sequence was cloned and crystal of the recombinant enzyme was obtained. However, quality of the crystal was not enough to solve structure so that the mechanism of substrate specificity of the endo-.-1,6-galactanase could not be elucidated.
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