2012 Fiscal Year Final Research Report
Functional analysis of magnesium channel family proteins in plant chloroplasts
| Project/Area Number |
22580123
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Bioproduction chemistry/Bioorganic chemistry
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| Research Institution | Kyoto Prefectural University |
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Principal Investigator |
ISHIJIMA Sumio 京都府立大学, 大学院・生命環境科学研究科, 准教授 (70184520)
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| Project Period (FY) |
2010 – 2012
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| Keywords | マグネシウムイオン / 膜輸送タンパク質 / 葉緑体 / リポソーム / シロイヌナズナ |
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| Research Abstract |
Arabidopsis thaliana AtMRS2-1, 10 and 11 proteins may play a role in Mg2+transport. These proteins were expressed in Escherichia coli, purified using an appropriate detergent, and reconstituted into liposomes. The reconstituted AtMRS2-10 transported Mg2+into liposomes, and its Mg2+transport was inhibited by Co2+, Ni2+and Al3+. The Mg2+transport through AtMRS2-1 and 11 was also observed with the reconstituted proteoliposomes.
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